Protein Variants | Comment | Organism |
---|---|---|
E224D | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Clostridium botulinum |
E262D | site-directed mutagenesis, the mutant shows a three-fold reduced activity compared to the wild-type enzyme | Clostridium botulinum |
E262Q | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Clostridium botulinum |
additional information | an insertion mutant moving Glu224 laterally by one residue is catalytically inactive | Clostridium botulinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics and kinetics of wild-type and mutant enzymes, overview | Clostridium botulinum | |
0.0086 | - |
SNAP-25 | pH 6.0, 37°C, mutant E262Q | Clostridium botulinum | |
0.0403 | - |
SNAP-25 | pH 6.0, 37°C, wild-type enzyme | Clostridium botulinum | |
0.043 | - |
SNAP-25 | pH 6.0, 37°C, mutant E262D | Clostridium botulinum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc endopeptidase with a active site zinc binding motif HEXXH containing His223, His227, Glu224, and Glu262, structure, overview, thw zinc ion might play a structural role in addition to its catalytic role, overview | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clostridium botulinum | the enzyme causes neuroparalysis by blocking neurotransmitter release at the neuromuscular junctions | ? | - |
? | |
SNAP-25 + H2O | Clostridium botulinum | i.e. synaptosomal associated protein of 25 kDa | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme causes neuroparalysis by blocking neurotransmitter release at the neuromuscular junctions | Clostridium botulinum | ? | - |
? | |
additional information | Glu224 and Glu262 are structurally essential for activity, structure-function relationship, overview | Clostridium botulinum | ? | - |
? | |
SNAP-25 + H2O | i.e. synaptosomal associated protein of 25 kDa | Clostridium botulinum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | comparison of wild-type and mutant tertiary structure by circular dichroism, overview | Clostridium botulinum |
Synonyms | Comment | Organism |
---|---|---|
BoNT/A LC | - |
Clostridium botulinum |
botulinum neurotoxin endopeptidase | - |
Clostridium botulinum |
botulinum neurotoxin type A light chain | - |
Clostridium botulinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium botulinum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
SNAP-25 | pH 6.0, 37°C, mutant E262Q | Clostridium botulinum | |
0.86 | - |
SNAP-25 | pH 6.0, 37°C, mutant E262D | Clostridium botulinum | |
2.134 | - |
SNAP-25 | pH 6.0, 37°C, wild-type enzyme | Clostridium botulinum | |
2.94 | - |
SNAP-25 | pH 6.0, 37°C, wild-type enzyme | Clostridium botulinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Clostridium botulinum |