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Literature summary for 3.4.24.69 extracted from

  • Kukreja, R.V.; Sharma, S.; Cai, S.; Singh, B.R.
    Role of two active site Glu residues in the molecular action of botulinum neurotoxin endopeptidase (2007), Biochim. Biophys. Acta, 1774, 213-222.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E224D site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Clostridium botulinum
E262D site-directed mutagenesis, the mutant shows a three-fold reduced activity compared to the wild-type enzyme Clostridium botulinum
E262Q site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Clostridium botulinum
additional information an insertion mutant moving Glu224 laterally by one residue is catalytically inactive Clostridium botulinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information thermodynamics and kinetics of wild-type and mutant enzymes, overview Clostridium botulinum
0.0086
-
SNAP-25 pH 6.0, 37°C, mutant E262Q Clostridium botulinum
0.0403
-
SNAP-25 pH 6.0, 37°C, wild-type enzyme Clostridium botulinum
0.043
-
SNAP-25 pH 6.0, 37°C, mutant E262D Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc endopeptidase with a active site zinc binding motif HEXXH containing His223, His227, Glu224, and Glu262, structure, overview, thw zinc ion might play a structural role in addition to its catalytic role, overview Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium botulinum the enzyme causes neuroparalysis by blocking neurotransmitter release at the neuromuscular junctions ?
-
?
SNAP-25 + H2O Clostridium botulinum i.e. synaptosomal associated protein of 25 kDa ?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme causes neuroparalysis by blocking neurotransmitter release at the neuromuscular junctions Clostridium botulinum ?
-
?
additional information Glu224 and Glu262 are structurally essential for activity, structure-function relationship, overview Clostridium botulinum ?
-
?
SNAP-25 + H2O i.e. synaptosomal associated protein of 25 kDa Clostridium botulinum ?
-
?

Subunits

Subunits Comment Organism
More comparison of wild-type and mutant tertiary structure by circular dichroism, overview Clostridium botulinum

Synonyms

Synonyms Comment Organism
BoNT/A LC
-
Clostridium botulinum
botulinum neurotoxin endopeptidase
-
Clostridium botulinum
botulinum neurotoxin type A light chain
-
Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Clostridium botulinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
SNAP-25 pH 6.0, 37°C, mutant E262Q Clostridium botulinum
0.86
-
SNAP-25 pH 6.0, 37°C, mutant E262D Clostridium botulinum
2.134
-
SNAP-25 pH 6.0, 37°C, wild-type enzyme Clostridium botulinum
2.94
-
SNAP-25 pH 6.0, 37°C, wild-type enzyme Clostridium botulinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Clostridium botulinum