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Literature summary for 3.4.24.69 extracted from
- Formation of a biologically active toxin complex of the binary Clostridium botulinum C2 toxin without cell membrane interaction (2006), Biochemistry, 45, 13361-13368.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme consists of two components, which are both secreted into the target cells or the cell culturemedium, respectively | Clostridium botulinum | - |
- |
| additional information | parasite enzyme component C2IIa forms ring-shaped heptamers, which bind to cell receptors and mediate the transport of component C2I into the cytosol of target cells. Receptor-bound C2IIa serves as a docking platform for C2I on the cell surface, following assembly of C2I, the toxin complex is taken up via receptor-mediated endocytosis, and finally, C2IIa facilitates translocation of C2I from acidic endosomes into the cytosol, overview | Clostridium botulinum | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Clostridium botulinum | the cytopathic effect of C2I-C2IIa toxin, e.g. on human HeLa or colon cancer Caco-2 cells, or Vero cells, is higher for the single components compared to the complex | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the cytopathic effect of C2I-C2IIa toxin, e.g. on human HeLa or colon cancer Caco-2 cells, or Vero cells, is higher for the single components compared to the complex | Clostridium botulinum | ? | - |
? | |
| additional information | the enzyme is a binary toxin, which is composed of two separate proteins, the enzyme component C2I is an ADP-ribosyltransferase which modifies G-actin of eukaryotic cells, while the proteolytically activated binding/translocation component C2IIa forms ring-shaped heptamers, which bind to cell receptors and mediate the transport of C2I into the cytosol of target cells. Receptor-bound C2IIa serves as a docking platform for C2I on the cell surface, following assembly of C2I, the toxin complex is taken up via receptor-mediated endocytosis, and finally, C2IIa facilitates translocation of C2I from acidic endosomes into the cytosol, the preformed C2 toxin complex ADP-ribosylates actin in vitro and induces cell rounding, overview | Clostridium botulinum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the enzyme is a binary toxin, which is composed of two separate proteins, the enzyme component C2I and the component C2IIa, overview | Clostridium botulinum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C2 toxin | - |
Clostridium botulinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium botulinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Clostridium botulinum |
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Release 2023.1 (January 2023)
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