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Literature summary for 3.4.24.69 extracted from

  • Schmidt, J.J.; Stafford, R.G.; Bostian, K.A.
    Type A botulinum neurotoxin proteolytic activity: development of competitive inhibitors and implications for substrate specificity at the S1' binding subsite (1998), FEBS Lett., 435, 61-64.
    View publication on PubMed
Search for more literature for 3.4.24.69

Application

Application Comment Organism
medicine remarkably effective drug for treating a wide variety of muscle dysfunctions in humans Clostridium botulinum

Inhibitors

Inhibitors Comment Organism Structure
Ac-SNKTRIDEACQRATKML-NH2
-
 Clostridium botulinum
Ac-SNKTRIDEAN(D)CRATKML-NH2
-
 Clostridium botulinum
Ac-SNKTRIDEAN(D)QCRATKML-NH2
-
 Clostridium botulinum
Ac-SNKTRIDEANCRATKML-NH2
-
 Clostridium botulinum
Ac-SNKTRIDEANQCATKML-NH2
-
 Clostridium botulinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 Ac-SNKTRIDECNQRATKML-NH2 pH 7.3, 37°C Clostridium botulinum
0.59
-
 Ac-SNKTRIDCANQRATKML-NH2 pH 7.3, 37°C Clostridium botulinum
0.89
-
 Ac-SNKTRIDEANQRCTKML-NH2 pH 7.3, 37°C Clostridium botulinum
1.7
-
 Ac-SNKTRIDEANQRATKML-NH2 pH 7.3, 37°C Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc metalloprotease Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Ac-SNKTIDEANQRATKML-NH2 + H2O Clostridium botulinum synaptosomal protein Ac-SNKTIDEANQ + RATKML-NH2
-
 ?
additional information Clostridium botulinum most potent neurotoxin known ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-SNKTIDEANQRATKML-NH2 + H2O synaptosomal protein Clostridium botulinum Ac-SNKTIDEANQ + RATKML-NH2
-
 ?
Ac-SNKTRIDCANQRATKML-NH2 + H2O
-
 Clostridium botulinum Ac-SNKTRIDCANQ + RATKML-NH2
-
 ?
Ac-SNKTRIDEANQRATKML-NH2 + H2O
-
 Clostridium botulinum Ac-SNKTRIDEANQ + RATKML-NH2
-
 ?
Ac-SNKTRIDEANQRCTKML-NH2 + H2O
-
 Clostridium botulinum Ac-SNKTRIDEANQ + RCTKML-NH2
-
 ?
Ac-SNKTRIDECNQRATKML + H2O
-
 Clostridium botulinum ?
-
 ?
Ac-SNKTRIDECNQRATKML-NH2 + H2O
-
 Clostridium botulinum Ac-SNKTRIDECNQ + RATKML-NH2
-
 ?
additional information most potent neurotoxin known Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
botox A
-
 Clostridium botulinum
type A botulinum neurotoxin
-
 Clostridium botulinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
 Ac-SNKTRIDECNQRATKML pH 7.3, 37°C Clostridium botulinum
23
-
 Ac-SNKTRIDCANQRATKML-NH2 pH 7.3, 37°C Clostridium botulinum
31
-
 Ac-SNKTRIDEANQRCTKML-NH2 pH 7.3, 37°C Clostridium botulinum
47
-
 Ac-SNKTRIDEANQRATKML-NH2 pH 7.3, 37°C Clostridium botulinum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.004
-
 Ac-SNKTRIDEAN(D)CRATKML-NH2 pH 7.3, 37°C Clostridium botulinum
0.11
-
 Ac-SNKTRIDEANCRATKML-NH2 pH 7.3, 37°C Clostridium botulinum
0.22
-
 Ac-SNKTRIDEACQRATKML-NH2 pH 7.3, 37°C Clostridium botulinum
0.65
-
 Ac-SNKTRIDEAN(D)QCRATKML-NH2 pH 7.3, 37°C Clostridium botulinum
2
-
 Ac-SNKTRIDEANQCATKML-NH2 pH 7.3, 37°C Clostridium botulinum