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Literature summary for 3.4.24.69 extracted from

  • Schiavo, G.; Montecucco, C.
    Tetanus and botulism neurotoxins: isolation and assay (1995), Methods Enzymol., 248, 643-652.
    View publication on PubMed
Search for more literature for 3.4.24.69

Activating Compound

Activating Compound Comment Organism Structure
Proteases activation by rapid cleavage of MW 150000 polypeptide chain and generation of active di-chain neurotoxin Clostridium sp.
Proteases activation by rapid cleavage of MW 150000 polypeptide chain and generation of active di-chain neurotoxin Clostridium botulinum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol accumulates until bacterial lysis Clostridium sp. 5829
-
cytosol accumulates until bacterial lysis Clostridium botulinum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc the L-chain of BoNT/B is a form of zinc-endopeptidase Clostridium sp.
Zinc the L-chain of BoNT/B is a form of zinc-endopeptidase Clostridium botulinum
Zinc 0.8-1 gatom zinc/mol neurotoxin Clostridium sp.
Zinc 0.8-1 gatom zinc/mol neurotoxin Clostridium botulinum
Zinc zinc-dependent endopeptidase (serotype BoNT/B) Clostridium sp.
Zinc zinc-dependent endopeptidase (serotype BoNT/B) Clostridium botulinum
Zinc atom absorption spectroscopy Clostridium sp.
Zinc atom absorption spectroscopy Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Neuroexocytosis multi-subunit complex + H2O Clostridium sp. neurotoxin binds specifically to nerve cells, botulin neurotoxin-receptors are located on the motor neuron plasmalemma at neuromuscular junctions, neurotoxin binds via protein and lipid interaction, after binding it is internalized inside vesicles of unknown nature ?
-
 ?
Neuroexocytosis multi-subunit complex + H2O Clostridium botulinum neurotoxin binds specifically to nerve cells, botulin neurotoxin-receptors are located on the motor neuron plasmalemma at neuromuscular junctions, neurotoxin binds via protein and lipid interaction, after binding it is internalized inside vesicles of unknown nature ?
-
 ?
Neuroexocytosis multi-subunit complex + H2O Clostridium sp. causing flaccid paralysis, in contrast to spastic paralysis caused by EC 3.4.24.68, three functionally distinct domains: domain L blocks neuroexocytosis, domain HN governs cell penetration, domain HC responsible for neurospecific binding ?
-
 ?
Neuroexocytosis multi-subunit complex + H2O Clostridium botulinum causing flaccid paralysis, in contrast to spastic paralysis caused by EC 3.4.24.68, three functionally distinct domains: domain L blocks neuroexocytosis, domain HN governs cell penetration, domain HC responsible for neurospecific binding ?
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 ?
synaptobrevin + H2O Clostridium sp. i.e. VAMP ?
-
 ?
synaptobrevin + H2O Clostridium botulinum i.e. VAMP ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
 7 serologically different neurotoxin types: BoNT/A-G
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Clostridium sp.
-
-
-

Oxidation Stability

Oxidation Stability Organism
extremely sensitive to oxidants Clostridium botulinum

Purification (Commentary)

Purification (Comment) Organism
serotypes BoNT/A, B, E, C, D, F Clostridium botulinum

Storage Stability

Storage Stability Organism
-80°C, in 10 mM HEPES buffer, pH 7.2, 50 mM NaCl, after freezing in liquid N2, stable Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no hydrolysis of short peptides spanning the respective cleavage sites of the target proteins Clostridium sp. ?
-
 ?
additional information no hydrolysis of short peptides spanning the respective cleavage sites of the target proteins Clostridium botulinum ?
-
 ?
Neuroexocytosis multi-subunit complex + H2O neurotoxin binds specifically to nerve cells, botulin neurotoxin-receptors are located on the motor neuron plasmalemma at neuromuscular junctions, neurotoxin binds via protein and lipid interaction, after binding it is internalized inside vesicles of unknown nature Clostridium sp. ?
-
 ?
Neuroexocytosis multi-subunit complex + H2O neurotoxin binds specifically to nerve cells, botulin neurotoxin-receptors are located on the motor neuron plasmalemma at neuromuscular junctions, neurotoxin binds via protein and lipid interaction, after binding it is internalized inside vesicles of unknown nature Clostridium botulinum ?
-
 ?
Neuroexocytosis multi-subunit complex + H2O causing flaccid paralysis, in contrast to spastic paralysis caused by EC 3.4.24.68, three functionally distinct domains: domain L blocks neuroexocytosis, domain HN governs cell penetration, domain HC responsible for neurospecific binding Clostridium sp. ?
-
 ?
Neuroexocytosis multi-subunit complex + H2O causing flaccid paralysis, in contrast to spastic paralysis caused by EC 3.4.24.68, three functionally distinct domains: domain L blocks neuroexocytosis, domain HN governs cell penetration, domain HC responsible for neurospecific binding Clostridium botulinum ?
-
 ?
Proteins of neuroexocytosis apparatus + H2O
-
 Clostridium sp. ?
-
 ?
Proteins of neuroexocytosis apparatus + H2O
-
 Clostridium botulinum ?
-
 ?
Synaptobrevin + H2O i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B Clostridium sp. Hydrolyzed synaptobrevin
-
 ?
Synaptobrevin + H2O i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
Synaptobrevin + H2O hydrolyzed by serotypes BoNT/B Clostridium sp. Hydrolyzed synaptobrevin
-
 ?
Synaptobrevin + H2O hydrolyzed by serotypes BoNT/B Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
Synaptobrevin + H2O hydrolyzed by serotypes D, F or G Clostridium sp. Hydrolyzed synaptobrevin
-
 ?
Synaptobrevin + H2O hydrolyzed by serotypes D, F or G Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
Synaptobrevin + H2O carrying synaptobrevin/VAMP-2 Clostridium sp. Hydrolyzed synaptobrevin
-
 ?
Synaptobrevin + H2O carrying synaptobrevin/VAMP-2 Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
Synaptobrevin + H2O highly specific neurotoxins Clostridium sp. Hydrolyzed synaptobrevin
-
 ?
Synaptobrevin + H2O highly specific neurotoxins Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
synaptobrevin + H2O i.e. VAMP Clostridium sp. ?
-
 ?
synaptobrevin + H2O i.e. VAMP Clostridium botulinum ?
-
 ?
Synaptosome-associated protein + H2O serotype BoNT/A and E Clostridium sp. Hydrolyzed synaptosome-associated protein
-
 ?
Synaptosome-associated protein + H2O serotype BoNT/A and E Clostridium botulinum Hydrolyzed synaptosome-associated protein
-
 ?
Syntaxin + H2O serotype BoNT/C Clostridium sp. ?
-
 ?
Syntaxin + H2O serotype BoNT/C Clostridium botulinum ?
-
 ?

Subunits

Subunits Comment Organism
More synthesized as single-chain polypeptide of about MW 150000 Da, proteolytic activation yields 2-chain neurotoxin with N-terminal light (MW 50000 Da) and C-terminal heavy chains (MW 100000 Da) connected by single disulfide bonds Clostridium sp.
More synthesized as single-chain polypeptide of about MW 150000 Da, proteolytic activation yields 2-chain neurotoxin with N-terminal light (MW 50000 Da) and C-terminal heavy chains (MW 100000 Da) connected by single disulfide bonds Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium sp.
37
-
 assay at Clostridium botulinum