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Literature summary for 3.4.24.69 extracted from

  • Schiavo, G.; Benfenati, F.; Poulain, B.; Rossetto, O.; Polverino de Laureto, P.; DasGupta, B.R.; Montecucco, C.
    Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin (1992), Nature, 359, 832-835.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ala-Ser-Gln-Phe-Glu-Thr-Ser synthetic peptide containing cleavage site of synaptobrevin, inhibits toxin action on buccal ganglion of Aplysia californica, serotype BoNT/B, not A or E Clostridium botulinum
captopril serotype BoNT/B Clostridium botulinum
EDTA serotype BoNT/B Clostridium botulinum
Gln-Phe-Glu-Thr synthetic peptide containing cleavage site of synaptobrevin, inhibits toxin action on buccal ganglion of Aplysia californica, serotype BoNT/B, not A or E Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc activation requires reduction of interchain disulfide bond Clostridium botulinum
Zinc zinc-dependent endopeptidase (serotype BoNT/B) Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
synaptobrevin + H2O Clostridium botulinum
-
?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
serotypes BoNT/A, BoNT/B
-
Clostridium botulinum
-
BoNT/E
-

Reaction

Reaction Comment Organism Reaction ID
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates mechanism Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information catalytic activity requires reduction of the single interchain disulfide bond of the neurotoxin Clostridium botulinum ?
-
?
additional information activating protease activity is localized on light or L-chain of neurotoxin Clostridium botulinum ?
-
?
Synaptobrevin + H2O hydrolyzed by serotypes BoNT/B Clostridium botulinum Hydrolyzed synaptobrevin
-
?
Synaptobrevin + H2O serotype BoNT/B: cleavage at Gln76-Phe77 Clostridium botulinum Hydrolyzed synaptobrevin
-
?
Synaptobrevin + H2O no substrate of serotype BoNT/A or E Clostridium botulinum Hydrolyzed synaptobrevin
-
?
Synaptobrevin + H2O carrying synaptobrevin/VAMP-2 Clostridium botulinum Hydrolyzed synaptobrevin
-
?
Synaptobrevin + H2O carrying Val76 instead of Gln76 is not hydrolyzed by serotype BoNT/B Clostridium botulinum Hydrolyzed synaptobrevin
-
?
Synaptobrevin + H2O highly specific neurotoxins Clostridium botulinum Hydrolyzed synaptobrevin
-
?
synaptobrevin + H2O
-
Clostridium botulinum ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Clostridium botulinum