Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala-Ser-Gln-Phe-Glu-Thr-Ser | synthetic peptide containing cleavage site of synaptobrevin, inhibits toxin action on buccal ganglion of Aplysia californica, serotype BoNT/B, not A or E | Clostridium botulinum | |
captopril | serotype BoNT/B | Clostridium botulinum | |
EDTA | serotype BoNT/B | Clostridium botulinum | |
Gln-Phe-Glu-Thr | synthetic peptide containing cleavage site of synaptobrevin, inhibits toxin action on buccal ganglion of Aplysia californica, serotype BoNT/B, not A or E | Clostridium botulinum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | activation requires reduction of interchain disulfide bond | Clostridium botulinum | |
Zinc | zinc-dependent endopeptidase (serotype BoNT/B) | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
synaptobrevin + H2O | Clostridium botulinum | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
serotypes BoNT/A, BoNT/B | - |
Clostridium botulinum | - |
BoNT/E | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates | mechanism | Clostridium botulinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | catalytic activity requires reduction of the single interchain disulfide bond of the neurotoxin | Clostridium botulinum | ? | - |
? | |
additional information | activating protease activity is localized on light or L-chain of neurotoxin | Clostridium botulinum | ? | - |
? | |
Synaptobrevin + H2O | hydrolyzed by serotypes BoNT/B | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | serotype BoNT/B: cleavage at Gln76-Phe77 | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | no substrate of serotype BoNT/A or E | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | carrying synaptobrevin/VAMP-2 | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | carrying Val76 instead of Gln76 is not hydrolyzed by serotype BoNT/B | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | highly specific neurotoxins | Clostridium botulinum | Hydrolyzed synaptobrevin | - |
? | |
synaptobrevin + H2O | - |
Clostridium botulinum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium botulinum |