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Literature summary for 3.4.24.67 extracted from

  • Yasumasu, S.; Kawaguchi, M.; Ouchi, S.; Sano, K.; Murata, K.; Sugiyama, H.; Akema, T.; Iuchi, I.
    Mechanism of egg envelope digestion by hatching enzymes, HCE and LCE in medaka, Oryzias latipes (2010), J. Biochem., 148, 439-448.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
480
-
NPQVPQYPSKPQ pH 8.0, 30°C Oryzias latipes

Organism

Organism UniProt Comment Textmining
Oryzias latipes
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Oryzias latipes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. HCE cleaves the N-terminal region of ZI-1,2 and ZI-3, mainly the Pro-Xaa-Yaa repeat sequence of ZI-1,2 into hexapeptides, but not the site within a zona pellucida (ZP) domain that is considered to be the core structure of the egg envelope. The cleavage of the N-terminal regions results in swelling and softening of the envelope Oryzias latipes ?
-
?
NPQVPQYPSKPQ + H2O Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 43.7 nmol/30min/microgram enzyme Oryzias latipes NPQVPQ + YPSKPQ
-
?
NPSYPQNPSYPQ + H2O Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 81.7 nmol/30min/microgram enzyme Oryzias latipes NPSYPQ + NPSYPQ
-
?
GSKTPQDPTQQ + H2O location: Q39/D40 in ChgL (ZI-3). Specific activity: 28.5 nmol/30min/microgram enzyme Oryzias latipes GSKTPQ + DPTQQ
-
?
PGKNPNTPPIG + H2O location: N220/T221 in ChgH (ZI-1,2). Specific activity: 11.3 nmol/30min/microgram enzyme Oryzias latipes PGKNPN + TPPIG
-
?
SAPVVRTSQAV + H2O synthetic substrate generated from the 25 kDa product in hatching liquid with an inefficient cleavage site Oryzias latipes NPQVPQ + YPSKPQ
-
?

Synonyms

Synonyms Comment Organism
HCE
-
Oryzias latipes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Oryzias latipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Oryzias latipes