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Literature summary for 3.4.24.67 extracted from

  • Sano, K.; Inohaya, K.; Kawaguchi, M.; Yoshizaki, N.; Iuchi, I.; Yasumasu, S.
    Purification and characterization of zebrafish hatching enzyme - an evolutionary aspect of the mechanism of egg envelope digestion (2008), FEBS J., 275, 5934-5946.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Danio rerio

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, complete inhibition Danio rerio
additional information not inhibitory: diisopropylfluorophosphate, iodoacetic acid Danio rerio

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hatching liquid i.e. culture medium after embryos hatched out Danio rerio
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
900
-
-
Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isoform ZHE1 cleaves the peptide bonds at the C-terminal side of Arg, Tyr, Asn, Trp, Ala, Asp, Phe and Gly Danio rerio ?
-
?
casein + H2O
-
Danio rerio ?
-
?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide + H2O about 40% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Danio rerio ?
-
?
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin 7-amide + H2O about 8.5% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Danio rerio ?
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
Danio rerio benzyloxycarbonyl-Phe + Arg-4-methylcoumarin 7-amide
-
?
KLMLKAPEPF + H2O synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 7.6% of the rate with TVQQSDYLIK Danio rerio KLMLK + APEPF
-
?
NPQVPQYPSKPQ + H2O synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 17% of the rate with TVQQSDYLIK Danio rerio NPQVPQ + YPSKPQ
-
?
NPSYPQNPSYPQ + H2O synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 32% of the rate with TVQQSDYLIK Danio rerio NPSYPQ + NPSYPQ
-
?
PLPVRVEEVV + H2O synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 38% of the rate with TVQQSDYLIK Danio rerio PLPVR + VEEVV
-
?
succinyl-Ile-Ile-Trp-4-methylcoumarin 7-amide + H2O about 5.7% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Danio rerio ?
-
?
t-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumarin 7-amide + H2O about 9% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Danio rerio ?
-
?
TVQQSDYLIK + H2O synthetic peptide substrate designed according to cleavage site in egg envelope protein Danio rerio TVQQS + DYLIK
-
?
ZP2 + H2O zebrafish egg envelope glycoprotein, cleavage at Ser47/Asp48 and Arg66/Val67 in the unfertilized egg, at Gln79/Ala80 in the fertilized egg Danio rerio ?
-
?
ZP3 + H2O zebrafish egg envelope glycoprotein, cleavage at Gln79/Ala80 in the unfertilized egg, at Gln67/Ala68 in the fertilized egg Danio rerio ?
-
?

Synonyms

Synonyms Comment Organism
ZHE1
-
Danio rerio