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Literature summary for 3.4.24.67 extracted from

  • Yasumasu, S.; Yamagami, K.
    Choriolysin H (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 625-627.
No PubMed abstract available

Application

Application Comment Organism
degradation HCE dissolves the inner layer of the egg envelope to facilitate hatching of the embryo Oryzias latipes

Cloned(Commentary)

Cloned (Comment) Organism
-
Oryzias latipes

Inhibitors

Inhibitors Comment Organism Structure
antichoriolysin H inhibits both choriolytic activity and binding to the egg envelope, does not affect caseinolytic activity Oryzias latipes
EDTA inhibits caseinolytic or choriolytic activity at 1 mM Oryzias latipes
additional information not inhibited by DFP, iodoacetamide or iodoacetic acid Oryzias latipes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Oryzias latipes
Ca2+
-
Oryzias latipes
Cu2+ high concentrations reactivate EDTA-inactivated apoenzyme Oryzias latipes
Zn2+ low concentrations reactivate EDTA-inactivated apoenzyme Oryzias latipes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
-
Oryzias latipes

Organism

Organism UniProt Comment Textmining
Oryzias latipes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partly purified by cation-exchange column chromatography and gel filtration, two isoforms Oryzias latipes

Source Tissue

Source Tissue Comment Organism Textmining
embryo expression of the enzyme starts early in the embryonic development, i. e. at the late gastrula stage Oryzias latipes
-
hatching gland colocalized with choriolysin L in the secretory granules Oryzias latipes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Oryzias latipes ?
-
?
chorion + H2O
-
Oryzias latipes ?
-
?
Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O
-
Oryzias latipes ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Oryzias latipes

Synonyms

Synonyms Comment Organism
High choriolytic enzyme
-
Oryzias latipes
chorionase
-
Oryzias latipes
HCE
-
Oryzias latipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.7 for choriolytic and caseinolytic activity, respectively Oryzias latipes