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Literature summary for 3.4.24.67 extracted from
- Choriolysin H (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 625-627.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | HCE dissolves the inner layer of the egg envelope to facilitate hatching of the embryo | Oryzias latipes |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Oryzias latipes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antichoriolysin H | inhibits both choriolytic activity and binding to the egg envelope, does not affect caseinolytic activity | Oryzias latipes | |
| EDTA | inhibits caseinolytic or choriolytic activity at 1 mM | Oryzias latipes |  |
| additional information | not inhibited by DFP, iodoacetamide or iodoacetic acid | Oryzias latipes |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Oryzias latipes | |
| Ca2+ | - |
Oryzias latipes | |
| Cu2+ | high concentrations reactivate EDTA-inactivated apoenzyme | Oryzias latipes | |
| Zn2+ | low concentrations reactivate EDTA-inactivated apoenzyme | Oryzias latipes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
- |
Oryzias latipes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryzias latipes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partly purified by cation-exchange column chromatography and gel filtration, two isoforms | Oryzias latipes |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | expression of the enzyme starts early in the embryonic development, i. e. at the late gastrula stage | Oryzias latipes | - |
| hatching gland | colocalized with choriolysin L in the secretory granules | Oryzias latipes | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Oryzias latipes | ? | - |
? | |
| chorion + H2O | - |
Oryzias latipes | ? | - |
? | |
| Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O | - |
Oryzias latipes | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Oryzias latipes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| High choriolytic enzyme | - |
Oryzias latipes |
| chorionase | - |
Oryzias latipes |
| HCE | - |
Oryzias latipes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.7 | for choriolytic and caseinolytic activity, respectively | Oryzias latipes |
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Release 2022.2 (July 2022)
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