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Literature summary for 3.4.24.67 extracted from

  • Kawaguchi, M.; Yasumasu, S.; Shimizu, A.; Hiroi, J.; Yoshizaki, N.; Nagata, K.; Tanokura, M.; Iuchi, I.
    Purification and gene cloning of Fundulus heteroclitus hatching enzyme. A hatching enzyme system composed of high choriolytic enzyme and low choriolytic enzyme is conserved between two different teleosts, Fundulus heteroclitus and medaka Oryzias latipes (2005), FEBS J., 272, 4315-4326.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Fundulus heteroclitus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
SDS-PAGE Fundulus heteroclitus

Organism

Organism UniProt Comment Textmining
Fundulus heteroclitus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, cation exchange column chromatography Fundulus heteroclitus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Fundulus heteroclitus
-
eye in stage 21 embryos, the HCE-expressing cells were located between two eye rudiments Fundulus heteroclitus
-
hatching gland
-
Fundulus heteroclitus
-
branchial arch in stage 25 embryos, strong expression of HCE found in branchial arches Fundulus heteroclitus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Fundulus heteroclitus ?
-
?
chorion + H2O moderate digestion by both HCE isoforms Fundulus heteroclitus ?
-
?
succinyl-Ala-Pro-Ala-4-methylcoumaryl-7-amide + H2O best substrate for both isoforms of HCE Fundulus heteroclitus ?
-
?
benzyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O preferred by HCE1 Fundulus heteroclitus ?
-
?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O preferred by HCE2 Fundulus heteroclitus ?
-
?

Synonyms

Synonyms Comment Organism
High choriolytic enzyme
-
Fundulus heteroclitus
HCE
-
Fundulus heteroclitus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
for both HCE isoforms Fundulus heteroclitus