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Literature summary for 3.4.24.67 extracted from

  • Kawaguchi, M.; Yasumasu, S.; Shimizu, A.; Hiroi, J.; Yoshizaki, N.; Nagata, K.; Tanokura, M.; Iuchi, I.
    Purification and gene cloning of Fundulus heteroclitus hatching enzyme. A hatching enzyme system composed of high choriolytic enzyme and low choriolytic enzyme is conserved between two different teleosts, Fundulus heteroclitus and medaka Oryzias latipes (2005), FEBS J., 272, 4315-4326.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Fundulus heteroclitus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 SDS-PAGE Fundulus heteroclitus

Organism

Organism UniProt Comment Textmining
Fundulus heteroclitus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, cation exchange column chromatography Fundulus heteroclitus

Source Tissue

Source Tissue Comment Organism Textmining
branchial arch in stage 25 embryos, strong expression of HCE found in branchial arches Fundulus heteroclitus
-
embryo
-
 Fundulus heteroclitus
-
eye in stage 21 embryos, the HCE-expressing cells were located between two eye rudiments Fundulus heteroclitus
-
hatching gland
-
 Fundulus heteroclitus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O preferred by HCE1 Fundulus heteroclitus ?
-
 ?
casein + H2O
-
 Fundulus heteroclitus ?
-
 ?
chorion + H2O moderate digestion by both HCE isoforms Fundulus heteroclitus ?
-
 ?
succinyl-Ala-Pro-Ala-4-methylcoumaryl-7-amide + H2O best substrate for both isoforms of HCE Fundulus heteroclitus ?
-
 ?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O preferred by HCE2 Fundulus heteroclitus ?
-
 ?

Synonyms

Synonyms Comment Organism
HCE
-
 Fundulus heteroclitus
High choriolytic enzyme
-
 Fundulus heteroclitus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 for both HCE isoforms Fundulus heteroclitus