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Literature summary for 3.4.24.67 extracted from

  • Yasumasu, S.; Katow, S.; Umino, Y.; Iuchi, I.; Yamagami, K.
    A unique proteolytic action of HCE, a constituent protease of a fish hatching enzyme: tight binding to its natural substrate, egg envelope (1989), Biochem. Biophys. Res. Commun., 162, 58-63.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Anti-HCE antibody inhibits choriolytic swelling and binding to chorion, no effect on caseinolytic activity Oryzias latipes

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Oryzias latipes
-
-

Organism

Organism UniProt Comment Textmining
Oryzias latipes
-
medaka, teleost fish, orange-red variety
-

Source Tissue

Source Tissue Comment Organism Textmining
blastula
-
Oryzias latipes
-
embryo prehatching embryo Oryzias latipes
-
hatching liquid i.e. culture medium of blastulae Oryzias latipes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Oryzias latipes ?
-
?
Chorion + H2O enzyme binds stoichiometrically to chorion (0.012 mg HCE/20 mg chorion) and remains bound to swollen chorion at pH 7.5 and is released at pH 10.2 Oryzias latipes Swollen chorion + ?
-
?
additional information swollen chorion Oryzias latipes ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Oryzias latipes