Any feedback?
Literature summary for 3.4.24.67 extracted from
- Purification and partial characterization of high choriolytic enzyme (HCE), a component of the hatching enzyme of the teleost, Oryzias latipes (1989), J. Biochem., 105, 204-211.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide as substrate, 0.8 mM ZnCl2 restores or 80 mM CuCl2 partially restores, not NiCl2, MgCl2 or CaCl2 | Oryzias latipes |  |
| additional information | no inhibition by monoiodoacetamide, monoiodoacetic acid or diisopropyl fluorophosphate | Oryzias latipes |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Oryzias latipes | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Calcium | 0.00286-0.00296 mg/mg protein, inductively coupled plasma emission analysis | Oryzias latipes | |
| Magnesium | 0.00063-0.00076 mg/mg protein, inductively coupled plasma emission analysis | Oryzias latipes | |
| additional information | copper barely detectable by atom absorption analysis and no cobalt, iron or manganese detected by inductively coupled plasma emission analysis | Oryzias latipes | |
| Zinc | 0.00127-0.00143 mg/mg protein, inductively coupled plasma emission analysis | Oryzias latipes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition of isomers HCE-1 and HCE-2 | Oryzias latipes |
| 20000 | - |
Oryzias latipes, deduced from sedimentation coefficient | Oryzias latipes |
| 23010 | - |
Oryzias latipes, HCE-1, estimation on the basis of amino acid composition | Oryzias latipes |
| 23470 | - |
Oryzias latipes, HCE-2, estimation on the basis of amino acid composition | Oryzias latipes |
| 24000 | - |
1 * 24000, Oryzias latipes, SDS-PAGE | Oryzias latipes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorion + H2O | Oryzias latipes | concerted action with other component of hatching enzyme, i.e. choriolysin L, EC 3.4.24.66 | ? | - |
? | |
| chorion + H2O | Oryzias latipes | embryo-secreted enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryzias latipes | - |
medaka, teleost fish, orange-red variety | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isomers: HCE-1 and HCE-2, separable by HPLC ion exchange chromatography | Oryzias latipes |
| from hatching liquid | Oryzias latipes |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blastula | - |
Oryzias latipes | - |
| embryo | embryo-secreted enzyme | Oryzias latipes | - |
| hatching liquid | i.e. culture medium of blastulae | Oryzias latipes | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryzias latipes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, in the presence of Ca2+ and Mg2+ in addition to Zn2+, 9 weeks | Oryzias latipes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzoyl-Arg 4-methylcoumarin 7-amide + H2O | hydrolysis at about 3% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| Benzyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O | hydrolysis at about 2% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| Chorion + H2O | - |
Oryzias latipes | Swollen chorion + ? | - |
? | |
| chorion + H2O | concerted action with other component of hatching enzyme, i.e. choriolysin L, EC 3.4.24.66 | Oryzias latipes | ? | - |
? | |
| chorion + H2O | embryo-secreted enzyme | Oryzias latipes | ? | - |
? | |
| additional information | exhibits choriolytic and proteolytic activity | Oryzias latipes | ? | - |
? | |
| additional information | N-tert-butoxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Glu-Lys-Lys 4-methylcoumarin 7-amide, succinyl-Gly-Pro 4-methylcoumarin 7-amide or Leu 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| N-tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | hydrolysis at about 12% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| Peptidyl 4-methylcoumarin 7-amides + H2O | i.e. MCA-peptides | Oryzias latipes | ? | - |
? | |
| Succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide + H2O | hydrolysis at about 13% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| Succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide + H2O | hydrolysis at about 45% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O | best substrate of peptidyl 4-methylcoumarin 7-amides | Oryzias latipes | Succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 24000, Oryzias latipes, SDS-PAGE | Oryzias latipes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Oryzias latipes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | 10 min, gradual decrease of choriolytic activity (10-30% loss) | Oryzias latipes |
| 25 | - |
4 days, in 50 mM Tris-HCl, 10 mM NaCl, pH 7.5, about 80% loss of choriolytic activity, 0.05% Brij-35 stabilizes, caseinolytic activity is less susceptible to long-term incubation | Oryzias latipes |
| 44 | - |
10 min, about 50% loss of choriolytic activity | Oryzias latipes |
| 50 | - |
10 min, about 90% loss of choriolytic activity | Oryzias latipes |
| 60 | - |
inactivation | Oryzias latipes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 10.2 | Oryzias latipes |
| 8 | - |
caseinolytic activity | Oryzias latipes |
| 8.7 | - |
choriolytic activity | Oryzias latipes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.9 | about 50% of maximal caseinolytic activity at pH 6.5 and 8.9 | Oryzias latipes |
| 7.9 | 9 | about 50% of maximal choriolytic activity at pH 7.9 and 9 | Oryzias latipes |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
