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Literature summary for 3.4.24.67 extracted from

  • Yasumasu, S.; Iuchi, I.; Yamagami, K.
    Purification and partial characterization of high choriolytic enzyme (HCE), a component of the hatching enzyme of the teleost, Oryzias latipes (1989), J. Biochem., 105, 204-211.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide as substrate, 0.8 mM ZnCl2 restores or 80 mM CuCl2 partially restores, not NiCl2, MgCl2 or CaCl2 Oryzias latipes
additional information no inhibition by monoiodoacetamide, monoiodoacetic acid or diisopropyl fluorophosphate Oryzias latipes

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Oryzias latipes
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc 0.00127-0.00143 mg/mg protein, inductively coupled plasma emission analysis Oryzias latipes
Calcium 0.00286-0.00296 mg/mg protein, inductively coupled plasma emission analysis Oryzias latipes
Magnesium 0.00063-0.00076 mg/mg protein, inductively coupled plasma emission analysis Oryzias latipes
additional information copper barely detectable by atom absorption analysis and no cobalt, iron or manganese detected by inductively coupled plasma emission analysis Oryzias latipes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition of isomers HCE-1 and HCE-2 Oryzias latipes
20000
-
Oryzias latipes, deduced from sedimentation coefficient Oryzias latipes
24000
-
1 * 24000, Oryzias latipes, SDS-PAGE Oryzias latipes
23010
-
Oryzias latipes, HCE-1, estimation on the basis of amino acid composition Oryzias latipes
23470
-
Oryzias latipes, HCE-2, estimation on the basis of amino acid composition Oryzias latipes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chorion + H2O Oryzias latipes concerted action with other component of hatching enzyme, i.e. choriolysin L, EC 3.4.24.66 ?
-
?
chorion + H2O Oryzias latipes embryo-secreted enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Oryzias latipes
-
medaka, teleost fish, orange-red variety
-

Purification (Commentary)

Purification (Comment) Organism
from hatching liquid Oryzias latipes
2 isomers: HCE-1 and HCE-2, separable by HPLC ion exchange chromatography Oryzias latipes

Source Tissue

Source Tissue Comment Organism Textmining
embryo embryo-secreted enzyme Oryzias latipes
-
blastula
-
Oryzias latipes
-
hatching liquid i.e. culture medium of blastulae Oryzias latipes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Oryzias latipes

Storage Stability

Storage Stability Organism
0°C, in the presence of Ca2+ and Mg2+ in addition to Zn2+, 9 weeks Oryzias latipes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information exhibits choriolytic and proteolytic activity Oryzias latipes ?
-
?
additional information N-tert-butoxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Glu-Lys-Lys 4-methylcoumarin 7-amide, succinyl-Gly-Pro 4-methylcoumarin 7-amide or Leu 4-methylcoumarin 7-amide Oryzias latipes ?
-
?
Succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide + H2O hydrolysis at about 13% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide Oryzias latipes ?
-
?
Succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide + H2O hydrolysis at about 45% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide Oryzias latipes ?
-
?
Chorion + H2O
-
Oryzias latipes Swollen chorion + ?
-
?
Peptidyl 4-methylcoumarin 7-amides + H2O i.e. MCA-peptides Oryzias latipes ?
-
?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O best substrate of peptidyl 4-methylcoumarin 7-amides Oryzias latipes Succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O hydrolysis at about 12% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide Oryzias latipes ?
-
?
Benzoyl-Arg 4-methylcoumarin 7-amide + H2O hydrolysis at about 3% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide Oryzias latipes ?
-
?
Benzyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O hydrolysis at about 2% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide Oryzias latipes ?
-
?
chorion + H2O concerted action with other component of hatching enzyme, i.e. choriolysin L, EC 3.4.24.66 Oryzias latipes ?
-
?
chorion + H2O embryo-secreted enzyme Oryzias latipes ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 24000, Oryzias latipes, SDS-PAGE Oryzias latipes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Oryzias latipes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, about 90% loss of choriolytic activity Oryzias latipes
25
-
4 days, in 50 mM Tris-HCl, 10 mM NaCl, pH 7.5, about 80% loss of choriolytic activity, 0.05% Brij-35 stabilizes, caseinolytic activity is less susceptible to long-term incubation Oryzias latipes
60
-
inactivation Oryzias latipes
44
-
10 min, about 50% loss of choriolytic activity Oryzias latipes
10 40 10 min, gradual decrease of choriolytic activity (10-30% loss) Oryzias latipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
caseinolytic activity Oryzias latipes
additional information
-
pI: 10.2 Oryzias latipes
8.7
-
choriolytic activity Oryzias latipes

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.9 about 50% of maximal caseinolytic activity at pH 6.5 and 8.9 Oryzias latipes
7.9 9 about 50% of maximal choriolytic activity at pH 7.9 and 9 Oryzias latipes