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Literature summary for 3.4.24.66 extracted from

  • Kawaguchi, M.; Yasumasu, S.; Shimizu, A.; Sano, K.; Iuchi, I.; Nishida, M.
    Conservation of the egg envelope digestion mechanism of hatching enzyme in euteleostean fishes (2010), FEBS J., 277, 4973-4987.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Fundulus heteroclitus
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Purification (Commentary)

Purification (Comment) Organism
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Fundulus heteroclitus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
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Fundulus heteroclitus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
EVLPLDNPPPA + H2O specific activity: 8.66 nmol/min/microgram protein Fundulus heteroclitus EVLPLD + NPPPA
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?
additional information cleavage sites of FHCE and FLCE on egg envelope subunit proteins are determined by comparing the N-terminal amino acid sequences of digests with the sequences deduced from the cDNAs for egg envelope subunit proteins. FHCE and FLCE cleave different sites of the subunit proteins. FLCE cleaves the inside of the zona pellucida domain, the core structure of egg envelope subunit protein, to completely digest the FHCE swollen envelope Fundulus heteroclitus ?
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?
additional information unfertilized egg envelope (UFE) is digested by one of the enzymes FHCE1/2 or FLCE Fundulus heteroclitus ?
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?
PSKRPEAPGVP + H2O specific activity: 0.56 nmol/min/microgram protein Fundulus heteroclitus PSKRPE + APGVP
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SVPVVRTSQAA + H2O specific activity: 19.5 nmol/min/microgram protein Fundulus heteroclitus SVPVVR + TSQAA
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VPFELRYPVPA + H2O specific activity: 10.36 nmol/min/microgram protein Fundulus heteroclitus VPFELR + YPVPA
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?
YPSKPQTPTET + H2O specific activity: 2.71 nmol/min/microgram protein Fundulus heteroclitus YPSKPQ + TPTET
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?

Synonyms

Synonyms Comment Organism
FLCE
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Fundulus heteroclitus
Low choriolytic enzyme
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Fundulus heteroclitus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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assay at Fundulus heteroclitus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Fundulus heteroclitus