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Literature summary for 3.4.24.66 extracted from

  • Yasumasu, S.; Yamagami, K.
    Choriolysin L (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 623-625.
No PubMed abstract available
Search for more literature for 3.4.24.66

Application

Application Comment Organism
degradation first choriolysin H swells the inner layer of egg envelope by limited digestion, and then choriolysin L solubilizes the swollen part of it completely Oryzias latipes

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibits at 1 mM Oryzias latipes
additional information not inhibited by DFP, iodoacetamide or iodoacetic acid Oryzias latipes

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ partly reactivates EDTA-inactivated apoenzyme Oryzias latipes
Zn2+ reactivates EDTA-inactivated apoenzyme Oryzias latipes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
-
 Oryzias latipes

Organism

Organism UniProt Comment Textmining
Oryzias latipes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partly purified by gel filtration and cation-exchange column Oryzias latipes

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Oryzias latipes
-
hatching gland colocalized with choriolysin H in the secretory granules Oryzias latipes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Oryzias latipes ?
-
 ?
chorion + H2O
-
 Oryzias latipes ?
-
 ?
Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O
-
 Oryzias latipes ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Oryzias latipes

Synonyms

Synonyms Comment Organism
chorionase
-
 Oryzias latipes
LCE
-
 Oryzias latipes
Low choriolytic enzyme
-
 Oryzias latipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 for caseinolytic activity Oryzias latipes