BRENDA - Enzyme Database show
show all sequences of 3.4.24.64

Reductive evolution of the mitochondrial processing peptidases of the unicellular parasites trichomonas vaginalis and giardia intestinalis

Smid, O.; Matuskova, A.; Harris, S.R.; Kucera, T.; Novotny, M.; Horvathova, L.; Hrdy, I.; Kutejova, E.; Hirt, R.P.; Embley, T.M.; Janata, J.; Tachezy, J.; PLoS Pathog. 4, e1000243 (2008)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
GPP is a metallopeptidase
Giardia intestinalis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
hydrogenosome
-
Trichomonas vaginalis
42566
-
mitosome
-
Giardia intestinalis
32047
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
GPP is a metallopeptidase
Giardia intestinalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44500
-
calculated
Giardia intestinalis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Giardia intestinalis
-
-
-
Trichomonas vaginalis
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity of the monomeric beta subunit of HPP is at the limit of detection for this assay (almost zero)
Trichomonas vaginalis
0.0023
-
approx., non-specified fluorescent peptide substrate based on the Trichomonas vaginalis adenylate kinase presequence, processing activity measured for the alpha subunit of HPP when associated with beta subunit of HPP
Trichomonas vaginalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
does cleave 4 out of 4 hydrogenosomal presequences, 1 out of 3 mitosomal substrates and 2 out of 3 mitochondrial substrates
700914
Trichomonas vaginalis
?
-
-
-
?
additional information
does cleave 4 out of 4 mitosomal substrates, does not cleave 0 out of 4 hydrogenosomal presequences and 0 out of 3 mitochondrial presequences
700914
Giardia intestinalis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
heterodimer
alpha and beta subunit of HPP, both subunits required to function most efficiently. Yeast alpha subunit of MPP does not interact with the Trichomonas beta subunit of HPP. Trichomonas alpha subunit of HPP is able to form a heterodimer with yeast beta subunit of MPP
Trichomonas vaginalis
monomer
beta subunit, functions efficiently as a single subunit monomer, data indicate that the monomeric enzyme evolved from an ancestral heterodimeric MPP comprising both an alpha and a beta subunit
Giardia intestinalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
GPP is a metallopeptidase
Giardia intestinalis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
hydrogenosome
-
Trichomonas vaginalis
42566
-
mitosome
-
Giardia intestinalis
32047
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
GPP is a metallopeptidase
Giardia intestinalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44500
-
calculated
Giardia intestinalis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity of the monomeric beta subunit of HPP is at the limit of detection for this assay (almost zero)
Trichomonas vaginalis
0.0023
-
approx., non-specified fluorescent peptide substrate based on the Trichomonas vaginalis adenylate kinase presequence, processing activity measured for the alpha subunit of HPP when associated with beta subunit of HPP
Trichomonas vaginalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
does cleave 4 out of 4 hydrogenosomal presequences, 1 out of 3 mitosomal substrates and 2 out of 3 mitochondrial substrates
700914
Trichomonas vaginalis
?
-
-
-
?
additional information
does cleave 4 out of 4 mitosomal substrates, does not cleave 0 out of 4 hydrogenosomal presequences and 0 out of 3 mitochondrial presequences
700914
Giardia intestinalis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
alpha and beta subunit of HPP, both subunits required to function most efficiently. Yeast alpha subunit of MPP does not interact with the Trichomonas beta subunit of HPP. Trichomonas alpha subunit of HPP is able to form a heterodimer with yeast beta subunit of MPP
Trichomonas vaginalis
monomer
beta subunit, functions efficiently as a single subunit monomer, data indicate that the monomeric enzyme evolved from an ancestral heterodimeric MPP comprising both an alpha and a beta subunit
Giardia intestinalis
Other publictions for EC 3.4.24.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733399
Teixeira
Processing peptidases in mitoc ...
Homo sapiens
Biochim. Biophys. Acta
1833
360-370
2013
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1
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733864
Endow
Stable complex formation of th ...
Arabidopsis thaliana
FEBS Lett.
587
2226-2231
2013
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2
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2
1
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1
1
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1
1
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733944
Mach
An advanced system of the mito ...
Trypanosoma brucei
Genome Biol. Evol.
5
860-875
2013
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1
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3
1
1
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7
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1
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733381
Mossmann
Processing of mitochondrial pr ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1819
1098-1106
2012
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-
-
-
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1
1
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1
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733730
Greene
Mitochondrial processing pepti ...
Homo sapiens
EMBO Rep.
13
378-385
2012
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1
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1
1
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2
2
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734654
Desy
Trypanosoma brucei has a canon ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
185
161-164
2012
-
-
-
-
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1
1
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1
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4
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1
1
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1
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1
1
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734853
Kwasniak
Proteolytic system of plant mi ...
Arabidopsis thaliana
Physiol. Plant.
145
187-195
2012
-
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-
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-
1
1
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1
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1
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1
1
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-
709853
Nagayama
Mitochondrial processing pepti ...
Dictyostelium discoideum
Microbiology
156
978-989
2010
-
-
1
-
1
-
-
-
3
-
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1
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3
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1
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1
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2
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1
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2
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2
2
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708076
Voegtle
Global analysis of the mitocho ...
Saccharomyces cerevisiae
Cell
139
428-439
2009
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1
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1
1
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709066
Naamati
Dual targeting of Nfs1 and dis ...
Saccharomyces cerevisiae
J. BIOL. CHEM.
284
30200-30208
2009
-
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2
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1
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1
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3
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1
1
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710508
Ohtsuka
Crystal structure of TTHA1264, ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Proteins
75
774-780
2009
-
-
1
1
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2
1
2
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3
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1
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2
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1
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2
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1
1
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696812
Nagayama
Antisense RNA inhibition of th ...
Dictyostelium discoideum
Biosci. Biotechnol. Biochem.
72
1836-1846
2008
-
-
1
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1
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3
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3
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1
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1
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700914
Smid
Reductive evolution of the mit ...
Giardia intestinalis, Trichomonas vaginalis
PLoS Pathog.
4
e1000243
2008
-
-
-
-
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-
1
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2
1
1
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8
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2
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2
2
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1
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2
1
1
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2
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2
2
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683617
Nishino
Spatial orientation of mitocho ...
Saccharomyces cerevisiae
J. Biochem.
141
889-895
2007
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2
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1
1
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2
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2
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1
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4
1
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2
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1
1
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4
1
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683664
Mukhopadhyay
Precursor protein is readily d ...
Homo sapiens, Saccharomyces cerevisiae
J. Biol. Chem.
282
37266-37275
2007
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2
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2
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2
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2
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4
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1
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2
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1
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1
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2
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2
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2
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2
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1
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2
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1
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1
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683686
Yoon
N-terminal iron-mediated self- ...
Homo sapiens
J. Biol. Inorg. Chem.
12
535-542
2007
-
-
-
-
-
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1
1
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2
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2
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683860
Osman
Prohibitins interact genetical ...
Saccharomyces cerevisiae
Mol. Biol. Cell
18
627-635
2007
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1
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4
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1
1
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2
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2
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669160
Nomura
Identification and reverse gen ...
Brugia malayi, Caenorhabditis elegans
J. Biochem.
139
967-979
2006
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2
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2
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1
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7
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3
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683997
Burri
Microsporidian mitosomes retai ...
Antonospora locustae, no activity in Encephalitozoon cuniculi
Proc. Natl. Acad. Sci. USA
103
15916-15920
2006
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1
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3
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9
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667491
Oshima
Recognition and processing of ...
Saccharomyces cerevisiae
Biochem. J.
385
755-761
2005
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667383
Janata
Substrate evokes translocation ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
316
211-217
2004
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6
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6
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1
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669838
Rudhe
Processing of the dual targete ...
Solanum tuberosum
J. Mol. Biol.
343
639-647
2004
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669840
Murcha
The N-terminal extension of pl ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae MY111-2, Solanum tuberosum
J. Mol. Biol.
344
443-454
2004
-
-
-
-
-
-
2
-
2
-
-
1
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
652393
Kitada
Determination of the cleavage ...
Rattus norvegicus, Saccharomyces cerevisiae
J. Biol. Chem.
278
1879-1885
2003
-
-
2
-
7
-
-
-
2
2
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
7
-
-
-
-
-
2
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649439
Gakh
Substrate binding changes conf ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
385
392-396
2001
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649576
Kitada
Glu191 and Asp195 in rat mitoc ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
287
594-599
2001
-
-
1
1
8
-
-
-
2
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
652133
Kojima
A proposed common structure of ...
Saccharomyces cerevisiae
J. Biol. Chem.
276
2115-2121
2001
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653929
Taylor
Crystal structures of mitochon ...
Saccharomyces cerevisiae
Structure
9
615-625
2001
-
-
1
1
6
-
-
-
-
1
1
-
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
6
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651746
Song
Importance of residues carboxy ...
Bos taurus
J. Biochem.
124
1045-1049
1998
-
-
-
-
-
-
1
17
1
-
-
-
-
1
-
-
1
-
-
-
-
-
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
17
1
-
-
-
-
-
-
1
-
-
-
-
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651890
Deng
Activation of a matrix process ...
Bos taurus
J. Biol. Chem.
273
20752-20757
1998
1
-
-
1
-
-
6
-
3
5
-
-
-
3
-
-
1
-
-
2
-
-
7
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
6
-
-
3
5
-
-
-
-
-
1
-
2
-
-
7
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
651893
Shimokata
Role of alpha-subunit of mitoc ...
Saccharomyces cerevisiae
J. Biol. Chem.
273
25158-25163
1998
-
-
1
-
11
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
11
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651907
Kojima
Cooperative formation of a sub ...
Saccharomyces cerevisiae
J. Biol. Chem.
273
32542-32546
1998
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651908
Kitada
Glutamate residues required fo ...
Rattus norvegicus, Saccharomyces cerevisiae
J. Biol. Chem.
273
32547-32553
1998
-
-
-
-
15
-
-
11
2
-
-
-
-
3
-
-
2
-
-
-
-
-
5
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
11
2
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
651520
Braun
The mitochondrial processing p ...
Neurospora sp., Rattus norvegicus, Saccharomyces cerevisiae, Solanum tuberosum, Triticum aestivum
Int. J. Biochem. Cell Biol.
29
1043-1045
1997
-
2
-
-
-
-
-
-
5
-
-
5
-
9
-
-
4
-
-
-
-
-
5
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
5
-
-
5
-
-
-
4
-
-
-
-
5
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31406
Brunner
Purification and characterizat ...
Neurospora crassa
Methods Enzymol.
248
717-728
1995
-
-
1
-
-
-
1
-
3
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
31407
Braun
The bifunctional cytochrome c ...
Solanum tuberosum, Spinacia oleracea, Triticum aestivum
J. Bioenerg. Biomembr.
27
423-436
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31408
Waltner
Conversion of a nonprocessed m ...
Rattus norvegicus
J. Biol. Chem.
270
26311-26317
1995
-
-
-
-
-
-
6
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31401
Saavedra-Alanis
Rat liver mitochondrial proces ...
Rattus norvegicus
J. Biol. Chem.
269
9284-9288
1994
-
-
1
-
-
-
-
-
2
-
3
-
-
6
-
-
1
-
-
2
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
1
-
2
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31402
Arretz
Characterization of the mitoch ...
Neurospora crassa
J. Biol. Chem.
269
4959-4967
1994
-
-
1
-
-
1
5
-
4
-
-
-
-
4
-
-
1
-
-
-
-
-
3
1
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
5
-
-
4
-
-
-
-
-
-
1
-
-
-
-
3
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
31404
Sjöling
A helical element in the C-ter ...
Spinacia oleracea
J. Biol. Chem.
269
32059-32062
1994
-
-
-
-
-
-
3
-
3
-
-
-
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31405
Bassham
Efficient but aberrant cleavag ...
Neurospora crassa
Eur. J. Biochem.
221
523-528
1994
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31382
Kalousek
Uniform nomenclature for the m ...
Neurospora crassa, Rattus norvegicus, Saccharomyces cerevisiae, Solanum tuberosum
Trends Biochem. Sci.
18
249
1993
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31399
Emmermann
The cytochrome c reductase int ...
Solanum tuberosum
Plant Physiol.
103
615-620
1993
-
-
-
-
-
-
2
-
3
1
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
1
-
-
-
-
-
-
-
1
-
-
5
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
31400
Emmermann
Characterization of the bifunc ...
More, Solanum tuberosum
J. Biol. Chem.
268
18936-18942
1993
-
-
1
-
-
2
-
-
2
1
-
-
-
5
-
-
-
1
-
1
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31403
Geli
Functional reconstitution in E ...
Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
90
6247-6251
1993
-
-
1
-
-
-
-
-
6
-
-
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31398
Eriksson
-
Mitochondrial processing prote ...
More, Solanum tuberosum, Spinacia oleracea
Biochim. Biophys. Acta
1140
208-214
1992
-
-
-
-
-
-
6
-
4
6
-
-
-
3
-
-
1
-
-
3
-
-
8
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
4
6
-
-
-
-
-
1
-
3
-
-
8
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
31314
Rawlings
Homologues of insulinase, a ne ...
Neurospora crassa, Saccharomyces cerevisiae
Biochem. J.
275
389-391
1991
-
-
-
-
-
-
-
-
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31395
Isaya
Cleavage of precursors by the ...
Rattus norvegicus
J. Cell Biol.
113
65-76
1991
-
-
1
-
-
-
-
-
1
-
2
1
-
4
-
-
1
-
-
1
1
1
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
1
1
1
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31396
Yang
The MAS-encoded processing pro ...
Saccharomyces cerevisiae
J. Biol. Chem.
266
6416-6423
1991
-
-
-
-
-
-
5
-
1
3
4
1
-
1
-
-
1
1
-
-
7
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
3
4
1
-
-
-
1
-
-
7
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31392
Kleiber
The general mitochondrial matr ...
More, Rattus norvegicus
Proc. Natl. Acad. Sci. USA
87
7978-7982
1990
-
-
1
-
-
-
1
-
2
1
2
2
-
2
-
-
1
-
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
1
2
2
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31393
Geli
The MAS-encoded processing pro ...
Saccharomyces cerevisiae
J. Biol. Chem.
265
19216-19222
1990
-
-
-
-
-
-
-
-
2
-
1
1
-
1
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31394
Schneider
Matrix processing peptidase of ...
More, Neurospora crassa
J. Biol. Chem.
265
9881-9887
1990
-
-
1
-
-
-
5
-
1
1
2
-
-
4
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
1
2
-
-
-
-
-
-
1
-
-
3
1
-
-
-
-
-
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31388
Hawlitschek
Mitochondrial protein import: ...
Neurospora crassa
Cell
53
795-806
1988
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6
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31389
Yang
Import of proteins into yeast ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae D-273-10B
EMBO J.
7
3857-3862
1988
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2
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1
12
2
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31390
Jensen
Import of proteins into yeast ...
Saccharomyces cerevisiae
EMBO J.
7
3863-3871
1988
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3
1
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31391
Witte
MAS1, a gene essential for yea ...
More, Saccharomyces cerevisiae
EMBO J.
7
1439-1447
1988
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2
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31386
Miura
A metalloprotease involved in ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
134
1151-1159
1986
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2
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4
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8
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1
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8
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31387
Kumamoto
Characterization of a mitochon ...
Bos taurus, Rattus norvegicus
J. Biochem.
100
247-254
1986
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2
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6
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1
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31383
Böhni
Processing of mitochondrial po ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae D-273-10B
Methods Enzymol.
97
311-323
1983
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2
4
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2
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4
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13
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1
13
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31384
McAda
A yeast mitochondrial chelator ...
Saccharomyces cerevisiae
Methods Enzymol.
97
337-344
1983
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3
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3
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1
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1
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31385
Böhni
Import of proteins into mitoch ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae D-273-10B
J. Biol. Chem.
258
4937-4943
1983
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4
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2
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4
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1
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16
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4
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2
2
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1
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1
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16
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1
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