Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.64 extracted from

  • Smid, O.; Matuskova, A.; Harris, S.R.; Kucera, T.; Novotny, M.; Horvathova, L.; Hrdy, I.; Kutejova, E.; Hirt, R.P.; Embley, T.M.; Janata, J.; Tachezy, J.
    Reductive evolution of the mitochondrial processing peptidases of the unicellular parasites trichomonas vaginalis and giardia intestinalis (2008), PLoS Pathog., 4, e1000243.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.64

Inhibitors

Inhibitors Comment Organism Structure
EDTA GPP is a metallopeptidase Giardia intestinalis

Localization

Localization Comment Organism GeneOntology No. Textmining
hydrogenosome
-
 Trichomonas vaginalis 42566
-
mitosome
-
 Giardia intestinalis 32047
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information GPP is a metallopeptidase Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44500
-
 calculated Giardia intestinalis

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-
Trichomonas vaginalis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity of the monomeric beta subunit of HPP is at the limit of detection for this assay (almost zero) Trichomonas vaginalis
0.0023
-
 approx., non-specified fluorescent peptide substrate based on the Trichomonas vaginalis adenylate kinase presequence, processing activity measured for the alpha subunit of HPP when associated with beta subunit of HPP Trichomonas vaginalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information does cleave 4 out of 4 hydrogenosomal presequences, 1 out of 3 mitosomal substrates and 2 out of 3 mitochondrial substrates Trichomonas vaginalis ?
-
 ?
additional information does cleave 4 out of 4 mitosomal substrates, does not cleave 0 out of 4 hydrogenosomal presequences and 0 out of 3 mitochondrial presequences Giardia intestinalis ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer alpha and beta subunit of HPP, both subunits required to function most efficiently. Yeast alpha subunit of MPP does not interact with the Trichomonas beta subunit of HPP. Trichomonas alpha subunit of HPP is able to form a heterodimer with yeast beta subunit of MPP Trichomonas vaginalis
monomer beta subunit, functions efficiently as a single subunit monomer, data indicate that the monomeric enzyme evolved from an ancestral heterodimeric MPP comprising both an alpha and a beta subunit Giardia intestinalis

Synonyms

Synonyms Comment Organism
GPP
-
 Giardia intestinalis
HPP
-
 Trichomonas vaginalis
mitochondrial processing peptidase
-
 Giardia intestinalis
mitochondrial processing peptidase
-
 Trichomonas vaginalis
MPP
-
 Giardia intestinalis
MPP
-
 Trichomonas vaginalis