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Literature summary for 3.4.24.64 extracted from

  • Nishino, T.G.; Kitano, K.; Kojima, K.; Ogishima, T.; Ito, A.; Kitada, S.
    Spatial orientation of mitochondrial processing peptidase and a preprotein revealed by fluorescence resonance energy transfer (2007), J. Biochem., 141, 889-895.
    View publication on PubMed
Search for more literature for 3.4.24.64

Protein Variants

Protein Variants Comment Organism
E73Q site-directed mutagenesis, active site mutant, forms dimers like the wild-type enzyme Saccharomyces cerevisiae
additional information construction of a EGFP fused to a yeast aconitase presequence and 7-dietylamino-3-(4'-maleimidyl phenyl)-4-methyl coumarin-labelled yeast MPPs for fluorescence resonance energy transfer analysis Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ MPP is a zinc-metalloendopeptidase Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
epidermal growth factor receptor preprotein + H2O Saccharomyces cerevisiae
-
 mature epidermal growth factor receptor + prepeptide of epidermal growth factor receptor
-
 ?
additional information Saccharomyces cerevisiae the enzyme specifically recognizes mitochondrial preproteins and removes their basic N-terminal signal prepeptides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 the amino acid residues on the C-terminal side of the cleavage site in the preprotein are orientated tail out from the large cavity of MPP and interact with the glycine-rich loop of the alpha-MPP subunit. Thus, MPP orientates preproteins at the specific cleft between the catalytic domain and the flexible glycine-rich loop, which seems to pinch the extended polypeptide, catalytic mechanism, overview Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epidermal growth factor receptor preprotein + H2O
-
 Saccharomyces cerevisiae mature epidermal growth factor receptor + prepeptide of epidermal growth factor receptor
-
 ?
epidermal growth factor receptor preprotein + H2O analysis of the fluorescence resonance energy transfer between EGFP fused to a yeast aconitase presequence and regiospecific 7-dietylamino-3-(4'-maleimidyl phenyl)-4-methyl coumarin-labelled yeast MPPs, overview Saccharomyces cerevisiae mature epidermal growth factor receptor + prepeptide of epidermal growth factor receptor
-
 ?
additional information the enzyme specifically recognizes mitochondrial preproteins and removes their basic N-terminal signal prepeptides Saccharomyces cerevisiae ?
-
 ?
additional information the enzyme specifically recognizes mitochondrial preproteins and removes their basic N-terminal signal prepeptides, overview Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
dimer MPP is a heterodimeric enzyme consisting of regulatory alpha-MPP and catalytic beta-MPP subunits, analysis of the fluorescence resonance energy transfer between EGFP fused to a yeast aconitase presequence and regiospecific 7-dietylamino-3-(4'-maleimidyl phenyl)-4-methyl coumarin-labelled yeast MPPs, plausible model of preEGFP associated with MPP constructed in silico, structure modeling, overview Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
MPP
-
 Saccharomyces cerevisiae