Cloned (Comment) | Organism |
---|---|
Mep1B, recombinant expression of homomeric meprin B used in human embryonic kidney (HEK293) cells, coexpression with meprin A (EC 3.4.24.18), recombinant expression of meprin B in MEDCK cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of meprin alphabeta knockout mice (alphabetaKO) which lack endogenous meprins, phenotype after CoCl2 exposure, overview | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc-dependent metalloprotease | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
osteosarcoma-9 + H2O | Mus musculus | OS-9, specific degradation by meprin beta | ? | - |
? | |
osteosarcoma-9 + H2O | Mus musculus C57BL/6 | OS-9, specific degradation by meprin beta | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q61847 | - |
- |
Mus musculus C57BL/6 | Q61847 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | meprin beta is activated using limited trypsin proteolysis | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant meprin B from HEK-293 cells | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
osteosarcoma-9 + H2O | OS-9, specific degradation by meprin beta | Mus musculus | ? | - |
? | |
osteosarcoma-9 + H2O | OS-9, purified recombinant human osteosarcoma-9 protein, specific degradation by meprin beta | Mus musculus | ? | - |
? | |
osteosarcoma-9 + H2O | OS-9, specific degradation by meprin beta | Mus musculus C57BL/6 | ? | - |
? | |
osteosarcoma-9 + H2O | OS-9, purified recombinant human osteosarcoma-9 protein, specific degradation by meprin beta | Mus musculus C57BL/6 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
meprin beta | - |
Mus musculus |
metalloproteinase meprin beta | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
metabolism | meprin metalloproteases play a role in the pathology of ischemia/reperfusion- (IR-) induced renal injury. The endoplasmic reticulum-associated protein, osteosarcoma-9 (OS-9), interacts with the C-terminal tail of meprin beta (two-hybrid system). OS-9 also interacts with the hypoxia inducible factor-1alpha (HIF-1alpha) and the prolyl-hydroxylase, proteins which mediate the cell's response to hypoxia. OS-9 proteins are most abundant in the cytosolic-enriched protein fraction of kidneys and does not occur in the brush border membrane-enriched fraction | Mus musculus |
physiological function | meprin B cleaves kidney OS-9 in ischemia/reperfusion | Mus musculus |