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Literature summary for 3.4.24.63 extracted from

  • Villa, J.P.; Bertenshaw, G.P.; Bond, J.S.
    Critical Amino Acids in the Active Site of Meprin Metalloproteinases for Substrate and Peptide Bond Specificity (2003), J. Biol. Chem., 278, 42545-42550.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.63

Activating Compound

Activating Compound Comment Organism Structure
Trypsin
-
 Mus musculus

Protein Variants

Protein Variants Comment Organism
K185Y site-directed mutagenesis Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00104
-
 gastrin 17 pH 7.5, 37°C, 150 mM NaCl Mus musculus
0.0185
-
 gastrin 17 pH 7.5, 37°C, 500 mM NaCl Mus musculus
0.02
-
 gastrin 17 pH 7.5, 37°C, 150 mM NaCl, mutant K185Y Mus musculus
0.0296
-
 gastrin 17 pH 7.5, 37°C, 500 mM NaCl, mutant K185Y Mus musculus
0.211
-
 cholecystokinin 8 sulfate pH 7.5, 37°C Mus musculus
0.22
-
 cholecystokinin 8 sulfate pH 7.5, 37°C, mutant K185Y Mus musculus
0.282
-
 alpha-MSH pH 7.5, 37°C, mutant K185Y Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-metalloendopeptidase Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 mouse
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-MSH + H2O
-
 Mus musculus ?
-
 ?
azocasein + H2O
-
 Mus musculus ?
-
 ?
cholecystokinin 8 sulfate + H2O
-
 Mus musculus ?
-
 ?
gastrin 17 + H2O
-
 Mus musculus ?
-
 ?
additional information neurotensin is no substrate Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
meprin metalloproteinase
-
 Mus musculus
mouse meprin beta
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.5
-
 gastrin 17 pH 7.5, 37°C, 500 mM NaCl, mutant K185Y Mus musculus
11
-
 gastrin 17 pH 7.5, 37°C, 150 mM NaCl Mus musculus
11.1
-
 gastrin 17 pH 7.5, 37°C, 500 mM NaCl Mus musculus
13.9
-
 cholecystokinin 8 sulfate pH 7.5, 37°C Mus musculus
15
-
 alpha-MSH pH 7.5, 37°C, mutant K185Y Mus musculus
15
-
 cholecystokinin 8 sulfate pH 7.5, 37°C, mutant K185Y Mus musculus
17.8
-
 gastrin 17 pH 7.5, 37°C, 150 mM NaCl, mutant K185Y Mus musculus