Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Trypsin | activation, 5-20fold | Mus musculus | |
Trypsin | only with larger substrates (azocasein or insulin B), not smaller substrates | Mus musculus | |
Trypsin | by limited proteolysis (2 h at 37°C, with tosyl-Phe chloromethyl ketone treated trypsin, in 20 mM Tris-HCl buffer, pH 7.5) | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | strong | Mus musculus | |
cysteine | - |
Mus musculus | |
EDTA | strong | Mus musculus | |
additional information | compound E-64; phosphoramidon, iodoacetic acid, 3,4-dichloroisocoumarin or soybean trypsin inhibitor | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | renal brush-border membrane | Mus musculus | 16020 | - |
additional information | cell surface endopeptidase | Mus musculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metalloendopeptidase according to inhibition profile | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition and secondary structure similar to meprin A | Mus musculus |
90000 | - |
x * 90000, mouse, SDS-PAGE under reducing or non-reducing conditions | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
inbred strain C3H/He | - |
Mus musculus | - |
male adults | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Mus musculus |
Purification (Comment) | Organism |
---|---|
papain-solubilized | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | brush border membrane | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.4763 mg azocasein/min mg protein | Mus musculus |
0.367 | 0.467 | Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly as substrate | Mus musculus |
1.82 | - |
insulin B-chain as substrate | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | poor substrate | Mus musculus | ? | - |
? | |
azocasein + H2O | mouse enzyme: poor substrate unless activated by trypsin treatment (i.e. latent azocaseinase activity) | Mus musculus | ? | - |
? | |
Insulin B-chain + H2O | 4 cleavage sites (2 major and 2 minor): His5-Leu6, Leu6-Cys(SO3-)7, Ala14-Leu15, Cys(SO3-)19-Gly20 | Mus musculus | ? | - |
? | |
Insulin B-chain + H2O | yielding at least 7 product peptides | Mus musculus | ? | - |
? | |
additional information | no substrates are nitrobradykinin | Mus musculus | ? | - |
? | |
Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly + H2O | synthetic octapeptide, derived from insulin B-chain | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 90000, mouse, SDS-PAGE under reducing or non-reducing conditions | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
t1/2: 2.5 min, less stable than meprin A | Mus musculus |