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Literature summary for 3.4.24.62 extracted from
- Proocytocin/neurophysin convertase from bovine neurohypophysis and corpus luteum secretory granules: complete purification, structure-function relationships, and competitive inhibitor (1989), Biochemistry, 28, 2705-2710.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | peptide I as substrate; strong, 2.5 mM | Bos taurus |  |
| EGTA | peptide I as substrate; strong, 2.5 mM | Bos taurus | |
| additional information | [D-Lys11]-pro-oxytocin/neurophysin(1-20), Gly-Gly-Lys-D-Arg-Ala-Val, Cys-Pro-Leu-Gly-Gly-Lys-D-Arg-Ala-Val; no inhibition by PMSF, pepstatin | Bos taurus | |
| p-chloromercuriphenylsulfonic acid | 2.5 mM; peptide I as substrate | Bos taurus | |
| PCMB | 2.5 mM; peptide I as substrate | Bos taurus | |
| [D-Arg12]-Pro-oxytocin/neurophysin(1-20) | kinetics; peptide I as substrate | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
peptide I | neurohypophyseal enzyme | Bos taurus | |
| 0.2 | - |
peptide I | corpus luteum | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| secretory granule | - |
Bos taurus | 30141 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
bovine, PAGE, 2 isoforms, MW 52000 and 58000 | Bos taurus |
| 58000 | - |
bovine, PAGE, 2 isoforms, MW 52000 and 58000 | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
cow | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Bos taurus |
| from purified vesicles | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| corpus luteum | - |
Bos taurus | - |
| hypophysis | pituitary neurosecretory granule | Bos taurus | - |
| neurohypophysis | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptide I + H2O | - |
Bos taurus | ? | - |
? | |
| Pro-oxytocin/neurophysin(1-20) + H2O | i.e. model substrate peptide I | Bos taurus | Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20) | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52000, SDS-PAGE, 1 * 58000, SDS-PAGE, 2 isoforms, MW 52000 and 58000 | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.9 or 6.9 (corpus luteum, different enzyme preparations) | Bos taurus |
| additional information | - |
pI: 6.9 and 7.1 (2 isoforms) | Bos taurus |
| 7 | - |
around, peptide I as substrate | Bos taurus |
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