BRENDA - Enzyme Database show
show all sequences of 3.4.24.62

Proocytocin/neurophysin convertase from bovine neurohypophysis and corpus luteum secretory granules: complete purification, structure-function relationships, and competitive inhibitor

Plevrakis, I.; Clamagirand, C.; Creminon, C.; Brakch, N.; Rholam, M.; Cohen, P.; Biochemistry 28, 2705-2710 (1989)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
peptide I as substrate; strong, 2.5 mM
Bos taurus
EGTA
peptide I as substrate; strong, 2.5 mM
Bos taurus
additional information
[D-Lys11]-pro-oxytocin/neurophysin(1-20), Gly-Gly-Lys-D-Arg-Ala-Val, Cys-Pro-Leu-Gly-Gly-Lys-D-Arg-Ala-Val; no inhibition by PMSF, pepstatin
Bos taurus
p-chloromercuriphenylsulfonic acid
2.5 mM; peptide I as substrate
Bos taurus
PCMB
2.5 mM; peptide I as substrate
Bos taurus
[D-Arg12]-Pro-oxytocin/neurophysin(1-20)
kinetics; peptide I as substrate
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
peptide I
neurohypophyseal enzyme
Bos taurus
0.2
-
peptide I
corpus luteum
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
secretory granule
-
Bos taurus
30141
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
bovine, PAGE, 2 isoforms, MW 52000 and 58000
Bos taurus
58000
-
bovine, PAGE, 2 isoforms, MW 52000 and 58000
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
cow
-
Purification (Commentary)
Commentary
Organism
affinity chromatography; from purified vesicles
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
corpus luteum
-
Bos taurus
-
hypophysis
pituitary neurosecretory granule
Bos taurus
-
neurohypophysis
-
Bos taurus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Pro-oxytocin/neurophysin(1-20) + H2O
i.e. model substrate peptide I
31375
Bos taurus
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 52000, SDS-PAGE, 1 * 58000, SDS-PAGE, 2 isoforms, MW 52000 and 58000
Bos taurus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 4.9 or 6.9 (corpus luteum, different enzyme preparations); pI: 6.9 and 7.1 (2 isoforms)
Bos taurus
7
-
around, peptide I as substrate
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
peptide I as substrate; strong, 2.5 mM
Bos taurus
EGTA
peptide I as substrate; strong, 2.5 mM
Bos taurus
additional information
[D-Lys11]-pro-oxytocin/neurophysin(1-20), Gly-Gly-Lys-D-Arg-Ala-Val, Cys-Pro-Leu-Gly-Gly-Lys-D-Arg-Ala-Val; no inhibition by PMSF, pepstatin
Bos taurus
p-chloromercuriphenylsulfonic acid
2.5 mM; peptide I as substrate
Bos taurus
PCMB
2.5 mM; peptide I as substrate
Bos taurus
[D-Arg12]-Pro-oxytocin/neurophysin(1-20)
kinetics; peptide I as substrate
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
peptide I
neurohypophyseal enzyme
Bos taurus
0.2
-
peptide I
corpus luteum
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
secretory granule
-
Bos taurus
30141
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
bovine, PAGE, 2 isoforms, MW 52000 and 58000
Bos taurus
58000
-
bovine, PAGE, 2 isoforms, MW 52000 and 58000
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
affinity chromatography; from purified vesicles
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
corpus luteum
-
Bos taurus
-
hypophysis
pituitary neurosecretory granule
Bos taurus
-
neurohypophysis
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Pro-oxytocin/neurophysin(1-20) + H2O
i.e. model substrate peptide I
31375
Bos taurus
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 52000, SDS-PAGE, 1 * 58000, SDS-PAGE, 2 isoforms, MW 52000 and 58000
Bos taurus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 4.9 or 6.9 (corpus luteum, different enzyme preparations); pI: 6.9 and 7.1 (2 isoforms)
Bos taurus
7
-
around, peptide I as substrate
Bos taurus
Other publictions for EC 3.4.24.62
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668826
Rholam
-
Magnolysin ...
Bos taurus, Homo sapiens
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
1039-1042
2004
-
-
-
-
-
-
12
-
-
-
2
-
-
2
-
-
2
-
-
4
-
-
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
2
-
-
-
-
2
-
4
-
-
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
31376
Guillou
Evidence for the presence of p ...
Homo sapiens
J. Endocrinol.
142
345-352
1994
-
-
-
-
-
-
2
-
1
-
1
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
1
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31374
Brakch
Processing endoprotease recogn ...
Bos taurus
J. Biol. Chem.
264
15912-15916
1989
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
-
1
-
-
1
-
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
-
1
-
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
31375
Plevrakis
Proocytocin/neurophysin conver ...
Bos taurus
Biochemistry
28
2705-2710
1989
-
-
-
-
-
-
6
2
1
-
2
-
-
1
-
-
1
-
-
3
-
-
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
1
-
2
-
-
-
-
1
-
3
-
-
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
31370
Cohen
Somatostatin-28 and pro-ocytoc ...
Bos taurus
Biochimie
70
17-23
1988
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31373
Creminon
Synthetic peptide substrates a ...
Bos taurus
J. Chromatogr.
440
439-448
1988
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
31371
Clamagirand
Partial purification and funct ...
Bos taurus
Biochemistry
26
6018-6023
1987
-
-
-
-
-
-
8
-
-
1
1
-
-
1
-
-
1
-
-
1
-
-
8
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
1
-
-
-
-
1
-
1
-
-
8
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
31372
Clamagirand
C-terminally extended ocytocin ...
Bos taurus
Biochem. Biophys. Res. Commun.
143
789-796
1987
-
-
-
-
-
-
5
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
1
-
1
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-