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show all sequences of 3.4.24.62

Partial purification and functional properties of an endoprotease from bovine neurosecretory granules cleaving proocytocin/neurophysin peptides at the basic amino acid doublet

Clamagirand, C.; Creminon, C.; Fahy, C.; Boussetta, H.; Cohen, P.; Biochemistry 26, 6018-6023 (1987)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
weak, 1 mM, peptide I as substrate
Bos taurus
Cd2+
weak, 0.1 mM, peptide I as substrate
Bos taurus
EDTA
peptide I as substrate; strong, 2.5 mM
Bos taurus
EGTA
peptide I as substrate; strong, 2.5 mM
Bos taurus
additional information
Mn2+, Mg2+, Cu2+; no inhibition by PMSF, pepstatin
Bos taurus
p-chloromercuriphenylsulfonic acid
2.5 mM; peptide I as substrate
Bos taurus
PCMB
2.5 mM; peptide I as substrate
Bos taurus
Zn2+
1 mM, peptide I as substrate
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
metalloendopeptidase
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
58000
bovine, gel filtration
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
from purified vesicles; partial
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
hypophysis
pituitary neurosecretory granule
Bos taurus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
No substrates are Nle11 or Nle12
31371
Bos taurus
?
-
-
-
-
additional information
No substrates are D-Lys11
31371
Bos taurus
?
-
-
-
-
additional information
No substrates are pro-oxytocin/neurophysin peptides (i.e. pro-OT/Np(1-20)) with D-Arg12
31371
Bos taurus
?
-
-
-
-
Pro-oxytocin/neurophysin(1-20) + H2O
synthetic eicosapeptide reproducing the entire sequence of amino-terminal domain of bovine proOc/Np precursor
31371
Bos taurus
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
31371
Bos taurus
-
Pro-oxytocin/neurophysin(1-20) + H2O
strictly selective for basic amino acid doublet Lys11-Arg12, cleavage site: Arg12-Ala13
31371
Bos taurus
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
31371
Bos taurus
-
Pro-oxytocin/neurophysin(1-20) + H2O
i.e. model substrate peptide I
31371
Bos taurus
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
31371
Bos taurus
-
[Ala10]-Pro-oxytocin/neurophysin(1-20) + H2O
i.e. peptide XI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20)
31371
Bos taurus
?
-
-
-
-
[Arg11,Lys12]-Pro-oxytocin/neurophysin(1-20) + H2O
i.e. peptide VI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20)
31371
Bos taurus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 6.9 and 7.2 (2 isoforms)
Bos taurus
7
-
around, peptide I as substrate
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
weak, 1 mM, peptide I as substrate
Bos taurus
Cd2+
weak, 0.1 mM, peptide I as substrate
Bos taurus
EDTA
peptide I as substrate; strong, 2.5 mM
Bos taurus
EGTA
peptide I as substrate; strong, 2.5 mM
Bos taurus
additional information
Mn2+, Mg2+, Cu2+; no inhibition by PMSF, pepstatin
Bos taurus
p-chloromercuriphenylsulfonic acid
2.5 mM; peptide I as substrate
Bos taurus
PCMB
2.5 mM; peptide I as substrate
Bos taurus
Zn2+
1 mM, peptide I as substrate
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
metalloendopeptidase
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
58000
bovine, gel filtration
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
from purified vesicles; partial
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
hypophysis
pituitary neurosecretory granule
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
No substrates are Nle11 or Nle12
31371
Bos taurus
?
-
-
-
-
additional information
No substrates are D-Lys11
31371
Bos taurus
?
-
-
-
-
additional information
No substrates are pro-oxytocin/neurophysin peptides (i.e. pro-OT/Np(1-20)) with D-Arg12
31371
Bos taurus
?
-
-
-
-
Pro-oxytocin/neurophysin(1-20) + H2O
synthetic eicosapeptide reproducing the entire sequence of amino-terminal domain of bovine proOc/Np precursor
31371
Bos taurus
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
31371
Bos taurus
-
Pro-oxytocin/neurophysin(1-20) + H2O
strictly selective for basic amino acid doublet Lys11-Arg12, cleavage site: Arg12-Ala13
31371
Bos taurus
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
31371
Bos taurus
-
Pro-oxytocin/neurophysin(1-20) + H2O
i.e. model substrate peptide I
31371
Bos taurus
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
31371
Bos taurus
-
[Ala10]-Pro-oxytocin/neurophysin(1-20) + H2O
i.e. peptide XI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20)
31371
Bos taurus
?
-
-
-
-
[Arg11,Lys12]-Pro-oxytocin/neurophysin(1-20) + H2O
i.e. peptide VI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20)
31371
Bos taurus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 6.9 and 7.2 (2 isoforms)
Bos taurus
7
-
around, peptide I as substrate
Bos taurus
Other publictions for EC 3.4.24.62
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668826
Rholam
-
Magnolysin ...
Bos taurus, Homo sapiens
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
1039-1042
2004
-
-
-
-
-
-
12
-
-
-
2
-
-
2
-
-
2
-
-
4
-
-
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
2
-
-
-
-
2
-
4
-
-
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
31376
Guillou
Evidence for the presence of p ...
Homo sapiens
J. Endocrinol.
142
345-352
1994
-
-
-
-
-
-
2
-
1
-
1
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
1
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31374
Brakch
Processing endoprotease recogn ...
Bos taurus
J. Biol. Chem.
264
15912-15916
1989
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
-
1
-
-
1
-
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
-
1
-
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
31375
Plevrakis
Proocytocin/neurophysin conver ...
Bos taurus
Biochemistry
28
2705-2710
1989
-
-
-
-
-
-
6
2
1
-
2
-
-
1
-
-
1
-
-
3
-
-
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
1
-
2
-
-
-
-
1
-
3
-
-
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
31370
Cohen
Somatostatin-28 and pro-ocytoc ...
Bos taurus
Biochimie
70
17-23
1988
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31373
Creminon
Synthetic peptide substrates a ...
Bos taurus
J. Chromatogr.
440
439-448
1988
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
31371
Clamagirand
Partial purification and funct ...
Bos taurus
Biochemistry
26
6018-6023
1987
-
-
-
-
-
-
8
-
-
1
1
-
-
1
-
-
1
-
-
1
-
-
8
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
1
-
-
-
-
1
-
1
-
-
8
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
31372
Clamagirand
C-terminally extended ocytocin ...
Bos taurus
Biochem. Biophys. Res. Commun.
143
789-796
1987
-
-
-
-
-
-
5
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
1
-
1
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-