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Literature summary for 3.4.24.61 extracted from

  • Gluschankof, P.; Gomez, S.; Morel, A.; Cohen, P.
    Enzymes that process somatostatin precursors. A novel endoprotease that cleaves before the arginine-lysine doublet is involved in somatostatin-28 convertase activity of rat brain cortex (1987), J. Biol. Chem., 262, 9615-9620.
    View publication on PubMed
Search for more literature for 3.4.24.61

Inhibitors

Inhibitors Comment Organism Structure
bestatin strong, 0.05 mM Rattus norvegicus
Ca2+ 1 mM Rattus norvegicus
Chelating agents strong Rattus norvegicus
Co2+ 1 mM; strong Rattus norvegicus
Cu2+ 1 mM; strong Rattus norvegicus
EDTA 10 mM Rattus norvegicus
EGTA 10 mM Rattus norvegicus
additional information diisopropyl fluorophosphate (6 mM); Mg2+ (1 mM Rattus norvegicus
Zn2+ 1 mM; strong Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information [Tyr18]-pro-oxytocin/neurophysin-(1-18)-NH2, Pro-Glu-D-Arg-Lys-Tyr-NH2, Pro-Glu-Arg-D-Lys-Tyr-NH2 Rattus norvegicus ?
-
 ?
Pro-Gln-Arg-Lys-Tyr-NH2 + H2O
-
 Rattus norvegicus Pro-Gln + Arg-Lys-Tyr-NH2
-
 ?
Pro-Glu-Arg-Arg-Tyr-NH2 + H2O
-
 Rattus norvegicus Pro-Glu + Arg-Arg-Tyr-NH2
-
 ?
Pro-Gly-Arg-Lys-Tyr-NH2 + H2O
-
 Rattus norvegicus Pro-Gly + Arg-Lys-Tyr-NH2
-
 ?
Somatostatin-28 + H2O cleavage on amino side of Arg-Lys doublet Rattus norvegicus Somatostatin-28-(13-28) + somatostatin-28-(1-12)
-
 ?
Somatostatin-28 + H2O cleavage site: Glu12-+-Arg13-Lys14, the term -+- depicts the point of cleavage Rattus norvegicus Somatostatin-28-(13-28) + somatostatin-28-(1-12)
-
 ?
Somatostatin-28 + H2O i.e. octacosapeptide Rattus norvegicus Somatostatin-28-(13-28) + somatostatin-28-(1-12)
-
 ?
[Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2 + H2O i.e. model peptide I Rattus norvegicus Pro-Arg-Glu + Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2
-
 ?
[Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2 + H2O i.e. Pro-Arg-Glu-Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2 Rattus norvegicus Pro-Arg-Glu + Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2
-
 ?

Synonyms

Synonyms Comment Organism
More endopeptidase of somatostatin-28 convertase complex Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 peptide I as substrate Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9 about half-maximal activity at pH 7.3 and about 90% of maximal activity at pH 9, peptide I as substrate Rattus norvegicus
8 9
-
 Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 3-15 h, 25% or less loss of activity Rattus norvegicus