Literature summary for 3.4.24.58 extracted from

Takeda, S.; Igarashi, T.; Mori, H.
Crystal structure of RVV-X: An example of evolutionary gain of specificity by ADAM proteinases (2007), FEBS Lett., 581, 5859-5864.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme by the sitting drop vapor diffusion method, mixing of 0.001 ml of protein solution with 0.001 ml of reservoir solution, containing 0.1M calcium acetate, 0.1M sodium cacodylate, 10% PEG 8000, pH 6.5, supplemented with one fifth volume of 10% PEG 3350, and equilibration against 1 ml of reservoir solution at 20°C, one week, cryoprotection with 15% 2-methyl-2,4-pentanediol in reservoir solution, X-ray diffraction structure determination and analysis at 2.9 A resolution, modeling	Daboia russelii

Crystallization (Comment)

Organism

purified enzyme by the sitting drop vapor diffusion method, mixing of 0.001 ml of protein solution with 0.001 ml of reservoir solution, containing 0.1M calcium acetate, 0.1M sodium cacodylate, 10% PEG 8000, pH 6.5, supplemented with one fifth volume of 10% PEG 3350, and equilibration against 1 ml of reservoir solution at 20°C, one week, cryoprotection with 15% 2-methyl-2,4-pentanediol in reservoir solution, X-ray diffraction structure determination and analysis at 2.9 A resolution, modeling

Daboia russelii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Daboia russelii	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	metalloproteinase	Daboia russelii

Organism

Organism	UniProt	Comment	Textmining
Daboia russelii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
further purification of the commercial venom enzyme by CM-resin chromatography in presence of N-[(2R)-2-(hydroxamidocarbonyllethyl)-4-methylpentanoyl]-L-tryptophan methylamide	Daboia russelii

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Daboia russelii	-
venom	-	Daboia russelii	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
factor X + H2O	docking model for factor X, overview	Daboia russelii	factor Xa + propeptide of factor Xa	-	?
additional information	Cys389 in the hyper-variable-region, residues 373-394, mediates the protein-protein interactions of the enzyme, Cys389 forms a disulfide bond with the C-terminal Cys133 of the enzyme's light-chain A	Daboia russelii	?	-	?

Subunits

Subunits	Comment	Organism
trimer	RVV-X is a heterotrimeric metalloproteinase with a mammalian ADAM-like heavy chain and two lectin-like light chains, it shows a hook-spanner-wrench-like architecture, in which the metalloproteinase/disintegrin region constitutes a hook, and the lectin-like domains constitute a handle	Daboia russelii

Synonyms

Synonyms	Comment	Organism
Russells viper venom factor X activator	-	Daboia russelii
RVV-X	-	Daboia russelii