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Literature summary for 3.4.24.58 extracted from

  • Furie, B.C.; Furie, B.
    Coagulant protein of Russells viper venom (1976), Methods Enzymol., 45, 191-205.
    View publication on PubMed
Search for more literature for 3.4.24.58

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 Factor X
-
 Daboia russelii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolute requirement for Daboia russelii
Ca2+ maximal concentration: 7 mM Daboia russelii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, Vipera russelli, SDS-PAGE in absence and presence of 2-mercaptoethanol Daboia russelii
124000
-
 Vipera russelli, sedimentation equilibrium centrifugation Daboia russelii

Organism

Organism UniProt Comment Textmining
Daboia russelii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Factor X + H2O activation by proteolytic cleavage of an Arg-Ile bond on the heavy chain of factor X which yields a small activation fragment with a molecular weight of 11000 and a large activation fragment (containing the active site serine) with a molecular weight of 44000 Daboia russelii A small activation fragment + a large activation fragment small activation fragment with a molecular weight of 11000 and a large activation fragment (containing the active site serine) with a molecular weight of 44000 ?
additional information no hydrolysis of: L-Phe-L-Phe, L-Phe-Gly, N-benzyloxycarbonyl-Gly-L-Phe, N-benzyloxycarbonyl-Gly-L-Phe-NH2, Gly-L-Phe-L-Phe, N-benzyloxycarbonyl-Glu-L-Tyr, benzoyl-L-Arg-NH2, L-Leu-Gly, Gly-L-Phe, Gly-L-Phe-NH2, hippuryl-L-Phe, B-chain of bovine insulin Daboia russelii ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60000, Vipera russelli, SDS-PAGE in absence and presence of 2-mercaptoethanol Daboia russelii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Daboia russelii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Daboia russelii

pH Range

pH Minimum pH Maximum Comment Organism
6 7.7 6.0: 25% of activity maximum, 7.7: activity maximum Daboia russelii