Cloned (Comment) | Organism |
---|---|
wild-type and E111Q glutathione S-transferase tagged human insulin-degrading enzyme are expressed in Escherichia coli BL21 (DE3) codon plus competent cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E111Q | inactive enzyme form | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Insulin | pH 7.4, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc metalloprotease | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme selectively degrades biologically important substrates associated with type 2 diabetes and Alzheimer's disease | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14735 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
insulin + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | the enzyme selectively degrades biologically important substrates associated with type 2 diabetes and Alzheimer's disease | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDE | - |
Homo sapiens |
Insulin-degrading enzyme | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
Insulin | pH 7.4, 37°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | ATP regulates insulin-degrading enzyme-dependent degradation of insulin, but the addition of Mg2+ abolishes the regulation | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
Insulin | pH 7.4, 37°C | Homo sapiens |