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Literature summary for 3.4.24.56 extracted from

  • Abdul-Hay, S.O.; Sahara, T.; McBride, M.; Kang, D.; Leissring, M.A.
    Identification of BACE2 as an avid beta-amyloid-degrading protease (2012), Mol. Neurodegener., 7, 46.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.56

Application

Application Comment Organism
medicine beta-site amyloid precursor protein-cleaving enzyme BACE2 overexpression in cultured cells lowers net amyloid beta levels to a greater extent than multiple, well-established amyloid beta-degrading proteases, including neprilysin and endothelinconverting enzyme-1, while showing comparable effectiveness to insulin degrading enzyme Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO cell Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 amyloid beta-peptide 1-42 pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.0028
-
 amyloid beta-peptide 1-40 pH not specified in the publication, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y5Z0 cf. EC 3.4.23.45
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amyloid beta-peptide 1-40 + H2O
-
 Homo sapiens ? cleavage occurs at peptide bonds Phe19-Phe20, Phe20-Ala21, and Leu34-Met35, with the latter cleavage site being the initial and principal one ?
amyloid beta-peptide 1-42 + H2O
-
 Homo sapiens ? cleavage occurs at peptide bonds Phe19-Phe20, Phe20-Ala21, and Leu34-Met35, with the latter cleavage site being the initial and principal one ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0004
-
 amyloid beta-peptide 1-42 pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.0022
-
 amyloid beta-peptide 1-40 pH not specified in the publication, temperature not specified in the publication Homo sapiens

General Information

General Information Comment Organism
physiological function beta-site amyloid precursor protein-cleaving enzyme BACE2 overexpression in cultured cells lowers net amyloid beta levels with comparable effectiveness to insulin degrading enzyme Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.178
-
 amyloid beta-peptide 1-42 pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.803
-
 amyloid beta-peptide 1-40 pH not specified in the publication, temperature not specified in the publication Homo sapiens