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Literature summary for 3.4.24.56 extracted from

  • Alper, B.J.; Rowse, J.W.; Schmidt, W.K.
    Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes (2009), Yeast, 26, 595-610.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged IDE in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E768A naturally occuring mutation of IDE Rattus norvegicus
Y248C naturally occuring mutation of IDE Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Rattus norvegicus
2-mercaptoethanol
-
Rattus norvegicus
4,7-phenanthroline
-
Rattus norvegicus
cysteine
-
Rattus norvegicus
DTT
-
Rattus norvegicus
EDTA
-
Rattus norvegicus
Insulin substrate inhibition Rattus norvegicus
additional information IDE is inhibited by metal chelators, thiol modifiers, inhibitors of cysteine protease activity and insulin, no inhibition by GTP and DMSO, poor inhibition by ATP Rattus norvegicus
NEM
-
Rattus norvegicus
PMSF
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.219
-
Abz-SEKKDNYIIKGV-nitroY-OH pH 9.2, 37°C, recombinant enzyme Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid beta40 + H2O Rattus norvegicus
-
amyloid beta40 peptide fragments
-
?
insulin + H2O Rattus norvegicus cleavage of the B-chain insulin peptide fragments
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged IDE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.22
-
pH 9.2, 37°C, purified recombinant His-tagged enzyme, substrate Abz-SEKKDNYIIKGV-nitroY-OH Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GFLRKGVQ-EDDnp + H2O
-
Rattus norvegicus ?
-
?
Abz-SEKKDNYIIKGV-nitroY-OH + H2O a substrate based on the polypeptide sequence of the yeast P2 a-factor mating propheromone Rattus norvegicus ?
-
?
amyloid beta40 + H2O
-
Rattus norvegicus amyloid beta40 peptide fragments
-
?
insulin + H2O cleavage of the B-chain Rattus norvegicus insulin peptide fragments
-
?

Synonyms

Synonyms Comment Organism
IDE
-
Rattus norvegicus
Insulin-degrading enzyme
-
Rattus norvegicus
More IDE belongs to the M16A enzyme family Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 49 temperature profile, recombinant enzyme, overview Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.8 9.2 pH profile, recombinant enzyme, overview Rattus norvegicus