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Literature summary for 3.4.24.56 extracted from

  • Amata, O.; Marino, T.; Russo, N.; Toscano, M.
    Human insulin-degrading enzyme working mechanism (2009), J. Am. Chem. Soc., 131, 14804-14811.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc-binding protease. The metal center in human IDE is chelated by His108, His112, and Glu189. The more external residues Tyr831, Glu111, Thr220, Gly221, Glu182, and Arg824 are also determined. Among these last residues, mainly Glu182, although not directly involved as metal ligand, plays a fundamental role in influencing metal recognition and binding by zinc proteins Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
insulin + H2O Homo sapiens
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insulin peptide fragments
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Degradation of insulin, glucagon and other polypeptides. No action on proteins reaction mechanism, active-site model, detailed overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CH3NH-Ala-Ala-Ala-CONHCH3 + H2O energetic profile of proteolysis mechanism of IDE Homo sapiens ?
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?
CH3NH-Leu-Tyr-Leu-CONHCH3 + H2O energetic profile of proteolysis mechanism of IDE Homo sapiens ?
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?
insulin + H2O
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Homo sapiens insulin peptide fragments
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?
additional information IDE shows catalytic activity toward two peptides of different length, simulating a portion of B chain of insulin, analysis by density functional theory method and the hybrid exchange-correlation functional B3LYP in gas phase and in the protein environment, modelling, reaction mechanism, overview. The proteolysis reaction is exothermic and proceeds quickly as the barrier in the rate-limiting step falls widely within the range of values expected for an enzymatic catalysis Homo sapiens ?
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?

Synonyms

Synonyms Comment Organism
IDE
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Homo sapiens
Insulin-degrading enzyme
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Homo sapiens
More IDE belongs to the protease M16A subfamily Homo sapiens