Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.53 extracted from

  • Takahashi, T.; Ohsaka, A.
    Purification and characterization of a proteinase in the venom of Trimeresurus flavoviridis. Complete separation of the enzyme from hemorrhagic activity (1970), Biochim. Biophys. Acta, 198, 293-307.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Benzyloxycarbonyl-Gly-Leu
-
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Leu-NH2
-
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Pro-Leu
-
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Pro-Leu-Gly
-
Protobothrops flavoviridis
Cd2+
-
Protobothrops flavoviridis
Cu2+
-
Protobothrops flavoviridis
cysteine
-
Protobothrops flavoviridis
EDTA
-
Protobothrops flavoviridis
Hg2+
-
Protobothrops flavoviridis
Ni2+
-
Protobothrops flavoviridis
PCMB
-
Protobothrops flavoviridis
Zn2+
-
Protobothrops flavoviridis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
22
-
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro
-
Protobothrops flavoviridis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information most active at ionic strength between 0.05 and 0.10 Protobothrops flavoviridis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
Trimeresurus flavoviridis, low speed sedimentation Protobothrops flavoviridis

Organism

Organism UniProt Comment Textmining
Protobothrops flavoviridis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Protobothrops flavoviridis

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Protobothrops flavoviridis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Protobothrops flavoviridis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O
-
Protobothrops flavoviridis Benzyloxycarbonyl-Gly-Pro + Leu-Gly-Pro
-
?
casein + H2O
-
Protobothrops flavoviridis hydrolyzed casein
-
?
additional information completely free from hemorrhagic acitivity Protobothrops flavoviridis ?
-
?
Oxidized insulin B-chain + H2O cleavage at Asn3-Gln4, His10-Leu11 and Ala14-Leu15 Protobothrops flavoviridis Hydrolyzed oxidized insulin B-chain
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Protobothrops flavoviridis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 about 30% of activity maximum at 30°C and 70°C Protobothrops flavoviridis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
pH 8.5, 5 min, enzyme retains full activity up to 45°C Protobothrops flavoviridis
60
-
pH 8.5, 5 min, complete loss of activity above Protobothrops flavoviridis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
casein Protobothrops flavoviridis

pH Range

pH Minimum pH Maximum Comment Organism
6 11 6: about 40% of activity maximum, 11: about 80% of activity maximum Protobothrops flavoviridis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
37°C, 1 h, about 40% loss of activity Protobothrops flavoviridis
7 9 37°C, 1 h, stable Protobothrops flavoviridis
11
-
37°C, 1 h, about 60% loss of activity Protobothrops flavoviridis