BRENDA - Enzyme Database show
show all sequences of 3.4.24.53

Purification and characterization of a proteinase in the venom of Trimeresurus flavoviridis. Complete separation of the enzyme from hemorrhagic activity

Takahashi, T.; Ohsaka, A.; Biochim. Biophys. Acta 198, 293-307 (1970)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Benzyloxycarbonyl-Gly-Leu
-
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Leu-NH2
-
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Pro-Leu
-
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Pro-Leu-Gly
-
Protobothrops flavoviridis
Cd2+
-
Protobothrops flavoviridis
Cu2+
-
Protobothrops flavoviridis
cysteine
-
Protobothrops flavoviridis
EDTA
-
Protobothrops flavoviridis
Hg2+
-
Protobothrops flavoviridis
Ni2+
-
Protobothrops flavoviridis
PCMB
-
Protobothrops flavoviridis
Zn2+
-
Protobothrops flavoviridis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
22
-
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro
-
Protobothrops flavoviridis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
most active at ionic strength between 0.05 and 0.10
Protobothrops flavoviridis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
Trimeresurus flavoviridis, low speed sedimentation
Protobothrops flavoviridis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Protobothrops flavoviridis
-
-
-
Purification (Commentary)
Commentary
Organism
-
Protobothrops flavoviridis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
venom
-
Protobothrops flavoviridis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Protobothrops flavoviridis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O
-
31297
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Pro + Leu-Gly-Pro
-
31297
Protobothrops flavoviridis
-
casein + H2O
-
31297
Protobothrops flavoviridis
hydrolyzed casein
-
31297
Protobothrops flavoviridis
-
additional information
completely free from hemorrhagic acitivity
31297
Protobothrops flavoviridis
?
-
-
-
-
Oxidized insulin B-chain + H2O
cleavage at Asn3-Gln4, His10-Leu11 and Ala14-Leu15
31297
Protobothrops flavoviridis
Hydrolyzed oxidized insulin B-chain
-
31297
Protobothrops flavoviridis
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Protobothrops flavoviridis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
70
about 30% of activity maximum at 30°C and 70°C
Protobothrops flavoviridis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
pH 8.5, 5 min, enzyme retains full activity up to 45°C
Protobothrops flavoviridis
60
-
pH 8.5, 5 min, complete loss of activity above
Protobothrops flavoviridis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
casein
Protobothrops flavoviridis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
11
6: about 40% of activity maximum, 11: about 80% of activity maximum
Protobothrops flavoviridis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
-
37°C, 1 h, about 40% loss of activity
Protobothrops flavoviridis
7
9
37°C, 1 h, stable
Protobothrops flavoviridis
11
-
37°C, 1 h, about 60% loss of activity
Protobothrops flavoviridis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Benzyloxycarbonyl-Gly-Leu
-
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Leu-NH2
-
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Pro-Leu
-
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Pro-Leu-Gly
-
Protobothrops flavoviridis
Cd2+
-
Protobothrops flavoviridis
Cu2+
-
Protobothrops flavoviridis
cysteine
-
Protobothrops flavoviridis
EDTA
-
Protobothrops flavoviridis
Hg2+
-
Protobothrops flavoviridis
Ni2+
-
Protobothrops flavoviridis
PCMB
-
Protobothrops flavoviridis
Zn2+
-
Protobothrops flavoviridis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
22
-
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro
-
Protobothrops flavoviridis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
most active at ionic strength between 0.05 and 0.10
Protobothrops flavoviridis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
Trimeresurus flavoviridis, low speed sedimentation
Protobothrops flavoviridis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Protobothrops flavoviridis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
venom
-
Protobothrops flavoviridis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Protobothrops flavoviridis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O
-
31297
Protobothrops flavoviridis
Benzyloxycarbonyl-Gly-Pro + Leu-Gly-Pro
-
31297
Protobothrops flavoviridis
-
casein + H2O
-
31297
Protobothrops flavoviridis
hydrolyzed casein
-
31297
Protobothrops flavoviridis
-
additional information
completely free from hemorrhagic acitivity
31297
Protobothrops flavoviridis
?
-
-
-
-
Oxidized insulin B-chain + H2O
cleavage at Asn3-Gln4, His10-Leu11 and Ala14-Leu15
31297
Protobothrops flavoviridis
Hydrolyzed oxidized insulin B-chain
-
31297
Protobothrops flavoviridis
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Protobothrops flavoviridis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
70
about 30% of activity maximum at 30°C and 70°C
Protobothrops flavoviridis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
pH 8.5, 5 min, enzyme retains full activity up to 45°C
Protobothrops flavoviridis
60
-
pH 8.5, 5 min, complete loss of activity above
Protobothrops flavoviridis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
casein
Protobothrops flavoviridis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
11
6: about 40% of activity maximum, 11: about 80% of activity maximum
Protobothrops flavoviridis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
-
37°C, 1 h, about 40% loss of activity
Protobothrops flavoviridis
7
9
37°C, 1 h, stable
Protobothrops flavoviridis
11
-
37°C, 1 h, about 60% loss of activity
Protobothrops flavoviridis
Other publictions for EC 3.4.24.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668855
Kawabata
-
Trimerelysin II ...
Protobothrops flavoviridis
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
704-705
2004
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31299
Kumasaka
Crystallization and preliminar ...
Protobothrops flavoviridis
J. Biochem.
117
929-930
1995
-
-
-
1
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31298
Takeya
Primary structure of H2-protei ...
Protobothrops flavoviridis
J. Biochem.
106
151-157
1989
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31297
Takahashi
Purification and characterizat ...
Protobothrops flavoviridis
Biochim. Biophys. Acta
198
293-307
1970
-
-
-
-
-
-
12
1
-
1
1
-
-
1
-
-
1
-
-
1
1
-
4
-
1
1
2
-
1
1
3
-
-
-
-
-
-
-
-
-
-
-
-
12
-
1
-
1
1
-
-
-
-
1
-
1
1
-
4
-
1
1
2
-
1
1
3
-
-
-
-
-
-
-