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Literature summary for 3.4.24.52 extracted from

  • Takeya, H.; Nishida, S.; Nishino, N.; Makinose, Y.; Omori-Satoh, T.; Nikai, T.; Sugihara, H.; Iwanaga, S.
    Primary structures of platelet aggregation inhibitors (disintegrins) autoproteolytically released from snake venom hemorrhagic metalloproteinases and new fluorogenic peptide substrates for these enzymes (1993), J. Biochem., 113, 473-483.
    View publication on PubMed
Search for more literature for 3.4.24.52

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
 2-Aminobenzoyl-Thr-Glu-Lys-Leu-Val-2,4-dinitroanilinoethylamide
-
 Protobothrops flavoviridis
0.013
-
 2-Aminobenzoyl-Phe-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide 2-aminobenzoyl-Gly-Phe-Arg-Leu-Leu-2,4-dinitroanilinoethylamide Protobothrops flavoviridis
0.014
-
 2-aminobenzoyl-Asn-Ala-Phe-Leu-Ala-2,4-dinitroanilinoethylamide
-
 Protobothrops flavoviridis
0.015
-
 2-Aminobenzoyl-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide
-
 Protobothrops flavoviridis
0.12
-
 2-Aminobenzoyl-Ala-Gly-Leu-Ala-2,4-dinitroanilinoethylamide
-
 Protobothrops flavoviridis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Protobothrops flavoviridis the major hemorrhagic component in the venom of Trimeresurus flavoviridis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Protobothrops flavoviridis
-
 Habu snake
-

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Protobothrops flavoviridis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Aminobenzoyl-Ala-Gly-Leu-Ala-2,4-dinitroanilinoethylamide + H2O peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate Protobothrops flavoviridis 2-Aminobenzoyl-Ala-Gly + Leu-Ala-2,4-dinitroanilinoethylamide
-
 ?
2-aminobenzoyl-Asn-Ala-Phe-Leu-Ala-2,4-dinitroanilinoethylamide + H2O
-
 Protobothrops flavoviridis ?
-
 ?
2-Aminobenzoyl-Asn-Ala-Pro-Leu-Ala-2,4-dinitroanilinoethylamide + H2O peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate Protobothrops flavoviridis 2-Aminobenzoyl-Asn-Ala-Pro + Leu-Ala-2,4-dinitroanilinoethylamide
-
 ?
2-Aminobenzoyl-Gly-Phe-Arg-Leu-Leu-2,4-dinitroanilinoethylamide + H2O peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate Protobothrops flavoviridis 2-Aminobenzoyl-Gly-Phe-Arg + Leu-Leu-2,4-dinitroanilinoethylamide
-
 ?
2-Aminobenzoyl-Phe-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide + H2O peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate Protobothrops flavoviridis 2-Aminobenzoyl-Phe-Ser-Pro + Met-Leu-2,4-dinitroanilinoethylamide
-
 ?
2-Aminobenzoyl-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide + H2O peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate Protobothrops flavoviridis 2-Aminobenzoyl-Ser-Pro + Met-Leu-2,4-dinitroanilinoethylamide
-
 ?
2-Aminobenzoyl-Thr-Glu-Lys-Leu-Val-2,4-dinitroanilinoethylamide + H2O peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate Protobothrops flavoviridis 2-Aminobenzoyl-Thr-Glu-Lys + Leu-Val-2,4-dinitroanilinoethylamide
-
 ?
Metalloproteinase HR1A + H2O MW 60000, bears autoproteolytic activity which is suppressed by more than 0.005 mM Ca2+ or 0.05 mM EDTA Protobothrops flavoviridis Hydrolyzed metalloproteinase HR1A fragment MW 32000 from COOH-terminus and low MW fragments ?
Metalloproteinase HR1B + H2O MW 60000, bears autoproteolytic activity which is suppressed by more than 5 mM Ca2+ or EDTA Protobothrops flavoviridis Hydrolyzed metalloproteinase HR1B fragment MW 34000 and low MW fragments ?
additional information 2-aminobenzoyl-Asn-Ala-Pro-Ser-Ala-2,4-dinitroanilinoethylamide, 2-aminobenzoyl-Ala-Thr-Asp-Ile-Val-2,4-dinitroanilinoethylamide, 2-aminobenzoyl-Gly-Phe-Arg-Leu-2,4-dinitroanilinoethylamide, 2-aminobenzoyl-Gly-Phe-Leu-Gly-Pro-2,4-dinitroanilinoethylamide Protobothrops flavoviridis ?
-
 ?
additional information the major hemorrhagic component in the venom of Trimeresurus flavoviridis Protobothrops flavoviridis ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Protobothrops flavoviridis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 2-aminobenzoyl-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide as substrate Protobothrops flavoviridis

pH Range

pH Minimum pH Maximum Comment Organism
6 10.5 half-maximal activity at pH 6 and 10.5, 2-aminobenzoyl-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide as substrate Protobothrops flavoviridis