Data extracted from this reference:
Cloned(Commentary)
DNA and amino acid sequence determination and analysis, phylogenetic tree
Ophiophagus hannah
Inhibitors
EDTA
complete inhibition
Ophiophagus hannah
additional information
no inhibition by PMSF
Ophiophagus hannah
Localization
extracellular
Ophiophagus hannah
Metals/Ions
additional information
metalloproteinase
Ophiophagus hannah
Molecular Weight [Da]
47344
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation
Ophiophagus hannah
50000
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation
Ophiophagus hannah
55000
gel filtration
Ophiophagus hannah
Natural Substrates/ Products (Substrates)
Fibrinogen + H2O
Ophiophagus hannah
human substrate, specific degradation of the alpha-chain, no activity with beta-chain and gamma-chain, the enzyme has a fibrinogenolytic activity and hemorrhagic activity in mice, overview
?
?
additional information
Ophiophagus hannah
the enzyme inhibits platelet aggregation induced by ADP, TMVA, and stejnulxin
?
?
Organism
Ophiophagus hannah
A3R0T9
king cobra
Posttranslational Modification
glycoprotein
ohagin contains four potential N-glycosylation sites
Ophiophagus hannah
Purification (Commentary)
native enzyme by gel filtration, ion exchange and heparin affinity chromatography
Ophiophagus hannah
Source Tissue
Specific Activity [micromol/min/mg]
additional information
hemorrhagic activity in mice, overview
Ophiophagus hannah
Substrates and Products (Substrate)
Fibrinogen + H2O
human substrate, specific degradation of the alpha-chain, no activity with beta-chain and gamma-chain, the enzyme has a fibrinogenolytic activity and hemorrhagic activity in mice, overview
684092
Ophiophagus hannah
?
?
Fibrinogen + H2O
specific degradation of the alpha-chain of the human substrate, while the beta- and gamma-chains are not hydrolyzed
684092
Ophiophagus hannah
?
?
additional information
the enzyme inhibits platelet aggregation induced by ADP, TMVA, and stejnulxin
684092
Ophiophagus hannah
?
?
Subunits
monomer
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation
Ophiophagus hannah
More
MALDI-TOF enzyme peptide mass fingerprinting, the enzyme contains a signal peptide, a proprotein, and a metalloproteinase, a disintegrin-like, and a cysteine-rich domain, overview
Ophiophagus hannah
Synonyms
ohagin
Ophiophagus hannah
Temperature Optimum [°C]
37
assay at
Ophiophagus hannah
pH Optimum
7.6
assay at
Ophiophagus hannah
pI Value
Ophiophagus hannah
sequence calculation
5.84
Cloned(Commentary) (protein specific)
DNA and amino acid sequence determination and analysis, phylogenetic tree
Ophiophagus hannah
Inhibitors (protein specific)
EDTA
complete inhibition
Ophiophagus hannah
additional information
no inhibition by PMSF
Ophiophagus hannah
Localization (protein specific)
extracellular
Ophiophagus hannah
Metals/Ions (protein specific)
additional information
metalloproteinase
Ophiophagus hannah
Molecular Weight [Da] (protein specific)
47344
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation
Ophiophagus hannah
50000
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation
Ophiophagus hannah
55000
gel filtration
Ophiophagus hannah
Natural Substrates/ Products (Substrates) (protein specific)
Fibrinogen + H2O
Ophiophagus hannah
human substrate, specific degradation of the alpha-chain, no activity with beta-chain and gamma-chain, the enzyme has a fibrinogenolytic activity and hemorrhagic activity in mice, overview
?
?
additional information
Ophiophagus hannah
the enzyme inhibits platelet aggregation induced by ADP, TMVA, and stejnulxin
?
?
Posttranslational Modification (protein specific)
glycoprotein
ohagin contains four potential N-glycosylation sites
Ophiophagus hannah
Purification (Commentary) (protein specific)
native enzyme by gel filtration, ion exchange and heparin affinity chromatography
Ophiophagus hannah
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
additional information
hemorrhagic activity in mice, overview
Ophiophagus hannah
Substrates and Products (Substrate) (protein specific)
Fibrinogen + H2O
human substrate, specific degradation of the alpha-chain, no activity with beta-chain and gamma-chain, the enzyme has a fibrinogenolytic activity and hemorrhagic activity in mice, overview
684092
Ophiophagus hannah
?
?
Fibrinogen + H2O
specific degradation of the alpha-chain of the human substrate, while the beta- and gamma-chains are not hydrolyzed
684092
Ophiophagus hannah
?
?
additional information
the enzyme inhibits platelet aggregation induced by ADP, TMVA, and stejnulxin
684092
Ophiophagus hannah
?
?
Subunits (protein specific)
monomer
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation
Ophiophagus hannah
More
MALDI-TOF enzyme peptide mass fingerprinting, the enzyme contains a signal peptide, a proprotein, and a metalloproteinase, a disintegrin-like, and a cysteine-rich domain, overview
Ophiophagus hannah
Temperature Optimum [°C] (protein specific)
37
assay at
Ophiophagus hannah
pH Optimum (protein specific)
7.6
assay at
Ophiophagus hannah
pI Value (protein specific)
Ophiophagus hannah
sequence calculation
5.84
Other publictions for EC 3.4.24.51
684092
Guo
Isolation and cloning of a met ...
Ophiophagus hannah
Toxicon
49
954-965
2007
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1
-
-
-
2
-
1
1
3
2
-
9
-
1
1
-
-
2
1
-
3
2
1
1
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
2
-
-
1
1
3
2
-
-
1
1
-
2
1
-
3
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
668853
Yamakawa
-
Ophiolysin ...
Ophiophagus hannah
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
699-701
2004
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-
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-
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2
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2
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1
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1
-
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1
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2
-
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-
-
-
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1
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-
-
-
-
-
-
-
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2
-
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-
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2
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1
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1
-
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2
-
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1
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-
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31292
Yamakawa
A protease in the venom of kin ...
Ophiophagus hannah
Toxicon
26
1145-1155
1988
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-
-
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5
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1
2
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1
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1
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1
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1
1
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1
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1
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5
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1
2
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1
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1
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1
1
1
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1
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