Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree | Ophiophagus hannah |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition | Ophiophagus hannah | |
additional information | no inhibition by PMSF | Ophiophagus hannah |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Ophiophagus hannah | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metalloproteinase | Ophiophagus hannah |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47344 | - |
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation | Ophiophagus hannah |
50000 | - |
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation | Ophiophagus hannah |
55000 | - |
gel filtration | Ophiophagus hannah |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | Ophiophagus hannah | human substrate, specific degradation of the alpha-chain, no activity with beta-chain and gamma-chain, the enzyme has a fibrinogenolytic activity and hemorrhagic activity in mice, overview | ? | - |
? | |
additional information | Ophiophagus hannah | the enzyme inhibits platelet aggregation induced by ADP, TMVA, and stejnulxin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ophiophagus hannah | A3R0T9 | king cobra | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | ohagin contains four potential N-glycosylation sites | Ophiophagus hannah |
Purification (Comment) | Organism |
---|---|
native enzyme by gel filtration, ion exchange and heparin affinity chromatography | Ophiophagus hannah |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Ophiophagus hannah | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
hemorrhagic activity in mice, overview | Ophiophagus hannah |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | human substrate, specific degradation of the alpha-chain, no activity with beta-chain and gamma-chain, the enzyme has a fibrinogenolytic activity and hemorrhagic activity in mice, overview | Ophiophagus hannah | ? | - |
? | |
Fibrinogen + H2O | specific degradation of the alpha-chain of the human substrate, while the beta- and gamma-chains are not hydrolyzed | Ophiophagus hannah | ? | - |
? | |
additional information | the enzyme inhibits platelet aggregation induced by ADP, TMVA, and stejnulxin | Ophiophagus hannah | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation | Ophiophagus hannah |
More | MALDI-TOF enzyme peptide mass fingerprinting, the enzyme contains a signal peptide, a proprotein, and a metalloproteinase, a disintegrin-like, and a cysteine-rich domain, overview | Ophiophagus hannah |
Synonyms | Comment | Organism |
---|---|---|
ohagin | - |
Ophiophagus hannah |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ophiophagus hannah |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Ophiophagus hannah |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ophiophagus hannah | sequence calculation | - |
5.84 |