BRENDA - Enzyme Database show
show all sequences of 3.4.24.51

A protease in the venom of king cobra (Ophiophagus hannah): purification, characterization and substrate specificity on oxidized insulin B-chain

Yamakawa, Y.; Omori-Satoh, T.; Toxicon 26, 1145-1155 (1988)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Ophiophagus hannah
formaldehyde
-
Ophiophagus hannah
Mn2+
-
Ophiophagus hannah
additional information
phenylmethylsulfonyl fluoride or N-ethylmaleimide are no inhibitors
Ophiophagus hannah
PMSF
-
Ophiophagus hannah
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
Mg2+ has no influence
Ophiophagus hannah
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
Ophiophagus hannah, gel filtration
Ophiophagus hannah
70000
-
1 * 70000, Ophiophagus hannah, SDS-PAGE
Ophiophagus hannah
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ophiophagus hannah
-
king cobra
-
Purification (Commentary)
Commentary
Organism
partial
Ophiophagus hannah
Source Tissue
Source Tissue
Commentary
Organism
Textmining
venom
-
Ophiophagus hannah
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Insulin B-chain + H2O
cleavage of Asn3-Gln, Gln4-His, His10-Leu, Ala14-Leu and Tyr16-Leu
31292
Ophiophagus hannah
Hydrolyzed insulin B-chain
-
31292
Ophiophagus hannah
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 70000, Ophiophagus hannah, SDS-PAGE
Ophiophagus hannah
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Ophiophagus hannah
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Ophiophagus hannah
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Ophiophagus hannah
formaldehyde
-
Ophiophagus hannah
Mn2+
-
Ophiophagus hannah
additional information
phenylmethylsulfonyl fluoride or N-ethylmaleimide are no inhibitors
Ophiophagus hannah
PMSF
-
Ophiophagus hannah
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
Mg2+ has no influence
Ophiophagus hannah
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
Ophiophagus hannah, gel filtration
Ophiophagus hannah
70000
-
1 * 70000, Ophiophagus hannah, SDS-PAGE
Ophiophagus hannah
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Ophiophagus hannah
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
venom
-
Ophiophagus hannah
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Insulin B-chain + H2O
cleavage of Asn3-Gln, Gln4-His, His10-Leu, Ala14-Leu and Tyr16-Leu
31292
Ophiophagus hannah
Hydrolyzed insulin B-chain
-
31292
Ophiophagus hannah
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 70000, Ophiophagus hannah, SDS-PAGE
Ophiophagus hannah
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Ophiophagus hannah
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Ophiophagus hannah
Other publictions for EC 3.4.24.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
684092
Guo
Isolation and cloning of a met ...
Ophiophagus hannah
Toxicon
49
954-965
2007
-
-
1
-
-
-
2
-
1
1
3
2
-
7
-
1
1
-
-
2
1
-
3
2
1
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
2
-
-
1
1
3
2
-
-
1
1
-
2
1
-
3
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
668853
Yamakawa
-
Ophiolysin ...
Ophiophagus hannah
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
699-701
2004
-
-
-
-
-
-
2
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
31292
Yamakawa
A protease in the venom of kin ...
Ophiophagus hannah
Toxicon
26
1145-1155
1988
-
-
-
-
-
-
5
-
-
1
2
-
-
1
-
-
1
-
-
1
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
1
2
-
-
-
-
1
-
1
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-