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Literature summary for 3.4.24.51 extracted from

  • Yamakawa, Y.; Omori-Satoh, T.
    A protease in the venom of king cobra (Ophiophagus hannah): purification, characterization and substrate specificity on oxidized insulin B-chain (1988), Toxicon, 26, 1145-1155.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Ophiophagus hannah
formaldehyde
-
Ophiophagus hannah
Mn2+
-
Ophiophagus hannah
additional information phenylmethylsulfonyl fluoride or N-ethylmaleimide are no inhibitors Ophiophagus hannah
PMSF
-
Ophiophagus hannah

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ has no influence Ophiophagus hannah

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
Ophiophagus hannah, gel filtration Ophiophagus hannah
70000
-
1 * 70000, Ophiophagus hannah, SDS-PAGE Ophiophagus hannah

Organism

Organism UniProt Comment Textmining
Ophiophagus hannah
-
king cobra
-

Purification (Commentary)

Purification (Comment) Organism
partial Ophiophagus hannah

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Ophiophagus hannah
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Insulin B-chain + H2O cleavage of Asn3-Gln, Gln4-His, His10-Leu, Ala14-Leu and Tyr16-Leu Ophiophagus hannah Hydrolyzed insulin B-chain
-
?

Subunits

Subunits Comment Organism
monomer 1 * 70000, Ophiophagus hannah, SDS-PAGE Ophiophagus hannah

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Ophiophagus hannah

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Ophiophagus hannah