A protease in the venom of king cobra (Ophiophagus hannah): purification, characterization and substrate specificity on oxidized insulin B-chain

Yamakawa, Y.; Omori-Satoh, T.; Toxicon 26, 1145-1155 (1988)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

EDTA

-

Ophiophagus hannah

formaldehyde

-

Ophiophagus hannah

Mn2+

-

Ophiophagus hannah

additional information

phenylmethylsulfonyl fluoride or N-ethylmaleimide are no inhibitors

Ophiophagus hannah

PMSF

-

Ophiophagus hannah

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

Insulin B-chain + H2O

cleavage of Asn3-Gln, Gln4-His, His10-Leu, Ala14-Leu and Tyr16-Leu

31292

Ophiophagus hannah

Hydrolyzed insulin B-chain

-

31292

Ophiophagus hannah

?

Inhibitors

Commentary

Organism

Structure

EDTA

-

Ophiophagus hannah

formaldehyde

-

Ophiophagus hannah

Mn2+

-

Ophiophagus hannah

additional information

phenylmethylsulfonyl fluoride or N-ethylmaleimide are no inhibitors

Ophiophagus hannah

PMSF

-

Ophiophagus hannah

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

Insulin B-chain + H2O

cleavage of Asn3-Gln, Gln4-His, His10-Leu, Ala14-Leu and Tyr16-Leu

31292

Ophiophagus hannah

Hydrolyzed insulin B-chain

-

31292

Ophiophagus hannah

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

684092

Guo

Isolation and cloning of a met ...

Ophiophagus hannah

Toxicon

49

954-965

2007

-

-

1

-

-

-

2

-

1

1

3

2

-

9

-

1

1

-

-

2

1

-

3

2

1

1

-

-

-

1

-

-

-

-

1

-

-

-

1

-

-

-

-

-

2

-

-

1

1

3

2

-

-

1

1

-

2

1

-

3

2

1

-

-

-

1

-

-

1

-

-

-

-

-

-

668853

Yamakawa

-

Ophiolysin ...

Ophiophagus hannah

Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press

1

699-701

2004

-

-

-

-

-

-

2

-

-

-

2

-

-

1

-

-

1

-

-

1

-

-

2

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

2

-

-

-

-

1

-

1

-

-

2

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

31292

Yamakawa

A protease in the venom of kin ...

Ophiophagus hannah

Toxicon

26

1145-1155

1988

-

-

-

-

-

-

5

-

-

1

2

-

-

1

-

-

1

-

-

1

-

-

1

1

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

-

1

2

-

-

-

-

1

-

1

-

-

1

1

1

-

-

-

1

-

-

-

-

-

-

-

-

-