BRENDA - Enzyme Database
show all sequences of 3.4.24.49

Bothropasin

Mandelbaum, F.R.; Assakura, M.T.; Reichl, A.P.; Serrano, S.M.; Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press 1, 658-659 (2004)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
local tissue damage after snakebite, causes hemorrhage followed by myonecrosis and arterial necrosis after intramuscular injection of doses of 0.02 mg in mice, myonecrosis is still observed with lower doses down to 0.001 mg
Bothrops jararaca
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
by 2 mM
Bothrops jararaca
DTT
by 2 mM
Bothrops jararaca
EDTA
by 2 mM
Bothrops jararaca
EGTA
by 2 mM
Bothrops jararaca
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37300
-
denatured and reduced protein in the presence of EDTA, SDS-PAGE, sedimentation equilibrium
Bothrops jararaca
48000
-
SDS-PAGE
Bothrops jararaca
49870
-
sedimentation equilibrium
Bothrops jararaca
Organism
Organism
UniProt
Commentary
Textmining
Bothrops jararaca
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
7fold
Bothrops jararaca
Source Tissue
Source Tissue
Commentary
Organism
Textmining
venom
-
Bothrops jararaca
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
casein + H2O
enzyme stabilized by CaCl2
668844
Bothrops jararaca
?
-
-
-
?
Fibrin + H2O
-
668844
Bothrops jararaca
?
-
-
-
?
fibrinogen
-
668844
Bothrops jararaca
?
-
-
-
?
fibronectin
-
668844
Bothrops jararaca
?
-
-
-
?
gelatin
-
668844
Bothrops jararaca
?
-
-
-
?
type I collagen
-
668844
Bothrops jararaca
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
-
Bothrops jararaca
Application (protein specific)
Application
Commentary
Organism
medicine
local tissue damage after snakebite, causes hemorrhage followed by myonecrosis and arterial necrosis after intramuscular injection of doses of 0.02 mg in mice, myonecrosis is still observed with lower doses down to 0.001 mg
Bothrops jararaca
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
by 2 mM
Bothrops jararaca
DTT
by 2 mM
Bothrops jararaca
EDTA
by 2 mM
Bothrops jararaca
EGTA
by 2 mM
Bothrops jararaca
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37300
-
denatured and reduced protein in the presence of EDTA, SDS-PAGE, sedimentation equilibrium
Bothrops jararaca
48000
-
SDS-PAGE
Bothrops jararaca
49870
-
sedimentation equilibrium
Bothrops jararaca
Purification (Commentary) (protein specific)
Commentary
Organism
7fold
Bothrops jararaca
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
venom
-
Bothrops jararaca
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
casein + H2O
enzyme stabilized by CaCl2
668844
Bothrops jararaca
?
-
-
-
?
Fibrin + H2O
-
668844
Bothrops jararaca
?
-
-
-
?
fibrinogen
-
668844
Bothrops jararaca
?
-
-
-
?
fibronectin
-
668844
Bothrops jararaca
?
-
-
-
?
gelatin
-
668844
Bothrops jararaca
?
-
-
-
?
type I collagen
-
668844
Bothrops jararaca
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
-
Bothrops jararaca
Other publictions for EC 3.4.24.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755598
Silva
Peptides derived from plasma ...
Bothrops jararaca
Toxicon
137
65-72
2017
-
-
-
-
-
-
-
-
-
1
-
-
-
7
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
735120
Srinivasa
Novel apigenin based small mol ...
Echis carinatus
PLoS ONE
9
e106364
2014
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
1
-
-
5
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734561
Paes Leme
Hemorrhagic activity of HF3, a ...
Bothrops jararaca
J. Proteome Res.
11
279-291
2012
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
7
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718413
Oliveira
New insights into the structur ...
Bothrops jararaca
Thromb. Haemost.
104
485-497
2010
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
9
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
9
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
701331
Oliveira
Simplified procedures for the ...
Bothrops jararaca
Toxicon
53
797-801
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701329
Muniz
The three-dimensional structur ...
Bothrops jararaca
Toxicon
52
807-816
2008
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670967
Carneiro
Venom production in long-term ...
Bothrops jararaca
Toxicon
47
87-94
2006
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668844
Mandelbaum
-
Bothropasin ...
Bothrops jararaca
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
658-659
2004
-
1
-
-
-
-
4
-
-
-
3
-
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
3
-
-
-
-
1
-
1
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
31290
Mandelbaum
Isolation and characterization ...
Bothrops jararaca
Toxicon
20
955-972
1982
-
-
-
-
-
1
3
-
-
1
3
-
-
3
-
-
1
-
-
3
-
-
2
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
1
3
-
-
-
-
1
-
3
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-