BRENDA - Enzyme Database
show all sequences of 3.4.24.49

Isolation and characterization of a proteolytic enzyme from the venom of the snake Bothrops jararaca (Jararaca)

Mandelbaum, F.R.; Reichel, A.P.; Assakura, M.T.; Toxicon 20, 955-972 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
Ca2+ stabilizes
Bothrops jararaca
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
complete inhibition
Bothrops jararaca
EGTA
complete inhibition
Bothrops jararaca
oxalate
85% inhibition, restorage of 85% of activity by removal of oxalate by addition of Ca2+
Bothrops jararaca
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM required for maximal activity
Bothrops jararaca
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37300
-
Bothrops jararaca, sedimentation equilibrium centrifugation in presence of EDTA
Bothrops jararaca
48000
-
x * 37300, Bothrops jararaca, SDS-PAGE in presence of EDTA, x * 48000, Bothrops jararaca, SDS-PAGE
Bothrops jararaca
49870
-
Bothrops jararaca, sedimentation equilibrium centrifugation in presence of 6 M guanidine-HCl and 0.1 M 2-mercaptoethanol
Bothrops jararaca
Organism
Organism
UniProt
Commentary
Textmining
Bothrops jararaca
-
jararaca snake
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Bothrops jararaca
Source Tissue
Source Tissue
Commentary
Organism
Textmining
venom
-
Bothrops jararaca
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
casein + H2O
endopeptidase
31290
Bothrops jararaca
hydrolyzed casein
-
31290
Bothrops jararaca
-
Insulin B-chain + H2O
cleavage of His5-Leu, His10-Leu, Ala14-Leu, Tyr16-Leu and Phe24-Phe
31290
Bothrops jararaca
Hydrolyzed insulin B-chain
-
31290
Bothrops jararaca
-
Subunits
Subunits
Commentary
Organism
monomer
x * 37300, Bothrops jararaca, SDS-PAGE in presence of EDTA, x * 48000, Bothrops jararaca, SDS-PAGE
Bothrops jararaca
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
assay at
Bothrops jararaca
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Bothrops jararaca
General Stability (protein specific)
General Stability
Organism
Ca2+ stabilizes
Bothrops jararaca
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
complete inhibition
Bothrops jararaca
EGTA
complete inhibition
Bothrops jararaca
oxalate
85% inhibition, restorage of 85% of activity by removal of oxalate by addition of Ca2+
Bothrops jararaca
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM required for maximal activity
Bothrops jararaca
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37300
-
Bothrops jararaca, sedimentation equilibrium centrifugation in presence of EDTA
Bothrops jararaca
48000
-
x * 37300, Bothrops jararaca, SDS-PAGE in presence of EDTA, x * 48000, Bothrops jararaca, SDS-PAGE
Bothrops jararaca
49870
-
Bothrops jararaca, sedimentation equilibrium centrifugation in presence of 6 M guanidine-HCl and 0.1 M 2-mercaptoethanol
Bothrops jararaca
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Bothrops jararaca
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
venom
-
Bothrops jararaca
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
casein + H2O
endopeptidase
31290
Bothrops jararaca
hydrolyzed casein
-
31290
Bothrops jararaca
-
Insulin B-chain + H2O
cleavage of His5-Leu, His10-Leu, Ala14-Leu, Tyr16-Leu and Phe24-Phe
31290
Bothrops jararaca
Hydrolyzed insulin B-chain
-
31290
Bothrops jararaca
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
x * 37300, Bothrops jararaca, SDS-PAGE in presence of EDTA, x * 48000, Bothrops jararaca, SDS-PAGE
Bothrops jararaca
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
assay at
Bothrops jararaca
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Bothrops jararaca
Other publictions for EC 3.4.24.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755598
Silva
Peptides derived from plasma ...
Bothrops jararaca
Toxicon
137
65-72
2017
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735120
Srinivasa
Novel apigenin based small mol ...
Echis carinatus
PLoS ONE
9
e106364
2014
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734561
Paes Leme
Hemorrhagic activity of HF3, a ...
Bothrops jararaca
J. Proteome Res.
11
279-291
2012
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1
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718413
Oliveira
New insights into the structur ...
Bothrops jararaca
Thromb. Haemost.
104
485-497
2010
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701331
Oliveira
Simplified procedures for the ...
Bothrops jararaca
Toxicon
53
797-801
2009
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701329
Muniz
The three-dimensional structur ...
Bothrops jararaca
Toxicon
52
807-816
2008
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670967
Carneiro
Venom production in long-term ...
Bothrops jararaca
Toxicon
47
87-94
2006
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1
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668844
Mandelbaum
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Bothropasin ...
Bothrops jararaca
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
658-659
2004
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31290
Mandelbaum
Isolation and characterization ...
Bothrops jararaca
Toxicon
20
955-972
1982
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