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Literature summary for 3.4.24.46 extracted from

  • Kress, L.F.; Catanese, J.J.
    Identification of the cleavage sites resulting from enzymatic inactivation of human antithrombin III by Crotalus adamanteus proteinase II in the the presence and absence of heparin (1981), Biochemistry, 20, 7432-7438.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
heparin activation, acceleration of antithrombin III inactivation, at a ratio of 6.7:1 (w/w) Crotalus adamanteus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Crotalus adamanteus

Organism

Organism UniProt Comment Textmining
Crotalus adamanteus
-
Eastern diamondback rattlesnake
-

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Crotalus adamanteus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Human antithrombin III + H2O in the presence of heparin non-inactivating cleavage in aminoterminal region at Glu37-Gln38 followed by inactivating cleavages in carboxyterminal region at Ala375-Ser376 and Ala378-Val379, in the absence of heparin inactivating cleavage in carboxyterminal region at Ser383-Ala384 Crotalus adamanteus Hydrolyzed and inactivated antithrombin III first occuring fragment in the presence of heparin is a still active antithrombin of MW 56000, followed by inactive fragments of MW 50000 and MW 5000, in the absence of heparin only inactive fragments of MW 56000 and MW 5000 occur ?
Human antithrombin III + H2O MW 60000, at a molar ratio of antithrombin III:enzyme of 40:1, in the presence and absence of heparin Crotalus adamanteus Hydrolyzed and inactivated antithrombin III first occuring fragment in the presence of heparin is a still active antithrombin of MW 56000, followed by inactive fragments of MW 50000 and MW 5000, in the absence of heparin only inactive fragments of MW 56000 and MW 5000 occur ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Crotalus adamanteus