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Literature summary for 3.4.24.45 extracted from
- Isolation and biochemical characterization of hemorrhagic toxin f from the venom of Crotalus atrox (western diamondback rattlesnake) (1984), Arch. Biochem. Biophys., 231, 309-319.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | casein as substrate | Crotalus atrox |  |
| EDTA | casein as substrate | Crotalus atrox | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc metalloproteinase, 1 mol zinc/mol enzyme | Crotalus atrox | |
| Zn2+ | essential for hemorrhagic and proteolytic activity | Crotalus atrox | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition, immunologically not related to hemorrhagic toxin a or c | Crotalus atrox |
| 64000 | - |
1 * 64000, Crotalus atrox, SDS-PAGE | Crotalus atrox |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Basement membranes surrounding capillaries + H2O | Crotalus atrox | produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crotalus atrox | - |
Western diamondback rattlesnake | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from lyophilized crude venom | Crotalus atrox |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Crotalus atrox | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Basement membranes surrounding capillaries + H2O | produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver | Crotalus atrox | ? | - |
? | |
| casein + H2O | - |
Crotalus atrox | ? | - |
? | |
| Dimethylcasein + H2O | - |
Crotalus atrox | ? | - |
? | |
| Fibrinogen + H2O | no coagulation | Crotalus atrox | Hydrolyzed fibrinogen | non-clottable products | ? | |
| Fibrinogen + H2O | cleaves gamma-chain, Aalpha and Bbeta-chains remain intact | Crotalus atrox | Hydrolyzed fibrinogen | non-clottable products | ? | |
| additional information | no hydrolysis of tosyl-L-Arg methyl ester or N-benzoyl-L-Arg ethyl ester | Crotalus atrox | ? | - |
? | |
| Oxidized insulin B-chain + H2O | cleaves Val2-Asn3, Gln4-His5, Leu6-Cys7, H10-Leu11, Ala14-Leu15, Tyr16-Leu17 bonds | Crotalus atrox | Hydrolyzed oxidized insulin B-chain | several peptides | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | in the presence of 2-mercaptoethanol | Crotalus atrox |
| monomer | 1 * 64000, Crotalus atrox, SDS-PAGE | Crotalus atrox |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Crotalus atrox |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 7.7 | Crotalus atrox |
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