Any feedback?
Literature summary for 3.4.24.44 extracted from
- Atrolysin E (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 674-676.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | one of the most hemorrhagic metalloproteinases of the P-II class in the venom, causes extensive hemorrhage in muscle tissue and extensive degeneration of capillaries, degradation of basement membrane proteins and surrounding stroma, its free disintegrin domain possesses platelet-aggregation inhibitory activity | Crotalus atrox |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| produced with a CMV expression vector in 293 cells | Crotalus atrox |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | inhibits proteolytic and hemorrhagic activities | Crotalus atrox |  |
| alpha2-Macroglobulin | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
| antihemorrhagins from serum of Crotalus atrox | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
| EDTA | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
| synthetic carboxyalkyl peptide | effective inhibitor | Crotalus atrox | |
| venom-derived pyroglutamyl-containing peptide | effective inhibitor | Crotalus atrox |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | low concentrations stabilize proteolytic activity | Crotalus atrox | |
| Zn2+ | can be replaced by Co2+, which does not significantly alter the proteolytic activity | Crotalus atrox | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25700 | - |
SDS-PAGE | Crotalus atrox |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crotalus atrox | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange chromatography and gel filtration | Crotalus atrox |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Crotalus atrox | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| basement membrane preperation from Engel-breth-Holm-Swarm tumor + H2O | great proteolytic activity | Crotalus atrox | ? | - |
? | |
| collagen type IV + H2O | - |
Crotalus atrox | ? | - |
? | |
| Dimethylcasein + H2O | - |
Crotalus atrox | ? | - |
? | |
| Fibrinogen + H2O | rapid cleavage of the Aalpha chain followed by the Bbeta and gamma chains | Crotalus atrox | ? | - |
? | |
| Laminin + H2O | - |
Crotalus atrox | ? | - |
? | |
| additional information | no cleavage of fibrin | Crotalus atrox | ? | - |
? | |
| Nidogen + H2O | - |
Crotalus atrox | ? | - |
? | |
| o-aminobenzoyl-Ala-Gly-Leu-Ala-nitrobenzylamide + H2O | - |
Crotalus atrox | ? | - |
? | |
| Oxidized insulin B-chain + H2O | - |
Crotalus atrox | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Crotalus atrox |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Hemorrhagic toxin e | - |
Crotalus atrox |
| HT-e | - |
Crotalus atrox |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
