BRENDA - Enzyme Database show
show all sequences of 3.4.24.44

Atrolysin E

Fox, J.W.; Bjarnason, J.B.; Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press 1, 674-676 (2004)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
one of the most hemorrhagic metalloproteinases of the P-II class in the venom, causes extensive hemorrhage in muscle tissue and extensive degeneration of capillaries, degradation of basement membrane proteins and surrounding stroma, its free disintegrin domain possesses platelet-aggregation inhibitory activity
Crotalus atrox
Cloned(Commentary)
Commentary
Organism
produced with a CMV expression vector in 293 cells
Crotalus atrox
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
inhibits proteolytic and hemorrhagic activities
Crotalus atrox
alpha2-Macroglobulin
inhibits proteolytic and hemorrhagic activities
Crotalus atrox
antihemorrhagins from serum of Crotalus atrox
inhibits proteolytic and hemorrhagic activities
Crotalus atrox
EDTA
inhibits proteolytic and hemorrhagic activities
Crotalus atrox
synthetic carboxyalkyl peptide
effective inhibitor
Crotalus atrox
venom-derived pyroglutamyl-containing peptide
effective inhibitor
Crotalus atrox
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
low concentrations stabilize proteolytic activity
Crotalus atrox
Zn2+
can be replaced by Co2+, which does not significantly alter the proteolytic activity
Crotalus atrox
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25700
-
SDS-PAGE
Crotalus atrox
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Crotalus atrox
-
-
-
Purification (Commentary)
Commentary
Organism
ion-exchange chromatography and gel filtration
Crotalus atrox
Source Tissue
Source Tissue
Commentary
Organism
Textmining
venom
-
Crotalus atrox
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
basement membrane preperation from Engel-breth-Holm-Swarm tumor + H2O
great proteolytic activity
668847
Crotalus atrox
?
-
-
-
?
collagen type IV + H2O
-
668847
Crotalus atrox
?
-
-
-
?
Dimethylcasein + H2O
-
668847
Crotalus atrox
?
-
-
-
?
Fibrinogen + H2O
rapid cleavage of the Aalpha chain followed by the Bbeta and gamma chains
668847
Crotalus atrox
?
-
-
-
?
Laminin + H2O
-
668847
Crotalus atrox
?
-
-
-
?
additional information
no cleavage of fibrin
668847
Crotalus atrox
?
-
-
-
?
Nidogen + H2O
-
668847
Crotalus atrox
?
-
-
-
?
o-aminobenzoyl-Ala-Gly-Leu-Ala-nitrobenzylamide + H2O
-
668847
Crotalus atrox
?
-
-
-
?
Oxidized insulin B-chain + H2O
-
668847
Crotalus atrox
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
-
Crotalus atrox
Application (protein specific)
Application
Commentary
Organism
medicine
one of the most hemorrhagic metalloproteinases of the P-II class in the venom, causes extensive hemorrhage in muscle tissue and extensive degeneration of capillaries, degradation of basement membrane proteins and surrounding stroma, its free disintegrin domain possesses platelet-aggregation inhibitory activity
Crotalus atrox
Cloned(Commentary) (protein specific)
Commentary
Organism
produced with a CMV expression vector in 293 cells
Crotalus atrox
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
inhibits proteolytic and hemorrhagic activities
Crotalus atrox
alpha2-Macroglobulin
inhibits proteolytic and hemorrhagic activities
Crotalus atrox
antihemorrhagins from serum of Crotalus atrox
inhibits proteolytic and hemorrhagic activities
Crotalus atrox
EDTA
inhibits proteolytic and hemorrhagic activities
Crotalus atrox
synthetic carboxyalkyl peptide
effective inhibitor
Crotalus atrox
venom-derived pyroglutamyl-containing peptide
effective inhibitor
Crotalus atrox
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
low concentrations stabilize proteolytic activity
Crotalus atrox
Zn2+
can be replaced by Co2+, which does not significantly alter the proteolytic activity
Crotalus atrox
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25700
-
SDS-PAGE
Crotalus atrox
Purification (Commentary) (protein specific)
Commentary
Organism
ion-exchange chromatography and gel filtration
Crotalus atrox
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
venom
-
Crotalus atrox
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
basement membrane preperation from Engel-breth-Holm-Swarm tumor + H2O
great proteolytic activity
668847
Crotalus atrox
?
-
-
-
?
collagen type IV + H2O
-
668847
Crotalus atrox
?
-
-
-
?
Dimethylcasein + H2O
-
668847
Crotalus atrox
?
-
-
-
?
Fibrinogen + H2O
rapid cleavage of the Aalpha chain followed by the Bbeta and gamma chains
668847
Crotalus atrox
?
-
-
-
?
Laminin + H2O
-
668847
Crotalus atrox
?
-
-
-
?
additional information
no cleavage of fibrin
668847
Crotalus atrox
?
-
-
-
?
Nidogen + H2O
-
668847
Crotalus atrox
?
-
-
-
?
o-aminobenzoyl-Ala-Gly-Leu-Ala-nitrobenzylamide + H2O
-
668847
Crotalus atrox
?
-
-
-
?
Oxidized insulin B-chain + H2O
-
668847
Crotalus atrox
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
-
Crotalus atrox
Other publictions for EC 3.4.24.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668847
Fox
-
Atrolysin E ...
Crotalus atrox
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
674-676
2004
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1
1
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6
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2
1
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1
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1
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1
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9
1
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1
1
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6
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2
1
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1
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1
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9
1
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649412
Shimokawa
Isolation, sequence analysis, ...
Crotalus atrox
Arch. Biochem. Biophys.
354
239-246
1998
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-
1
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5
-
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4
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-
1
-
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1
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1
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5
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1
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1
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-
-
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-
31259
Fox
Atrolysins: metalloproteinases ...
Crotalus atrox
Methods Enzymol.
248
368-387
1995
-
-
1
-
-
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6
-
-
2
1
1
-
1
-
1
1
-
-
1
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18
1
-
-
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-
1
-
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1
-
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6
-
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2
1
1
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1
1
-
1
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18
1
-
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-
1
-
-
-
-
-
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-
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-
31260
Bjarnason
Studies on the mechanism of he ...
Crotalus atrox
Biol. Chem. Hoppe-Seyler
369
121-129
1988
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-
-
-
-
-
2
-
-
1
1
-
-
1
-
-
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-
1
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6
1
1
-
-
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
2
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1
1
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-
1
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-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
31276
Bjarnason
Proteolytic specificity and co ...
Crotalus atrox
Biochemistry
22
3770-3778
1983
-
-
-
-
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2
-
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2
-
-
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2
-
-
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-
2
-
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5
-
1
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5
-
-
-
-
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-
-
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-
-
-
-
-
-
-
2
-
-
-
2
-
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2
-
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5
-
1
-
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5
-
-
-
-
-
-
-
-
-
-
31261
Bjarnason
Hemorrhagic toxins from Wester ...
Crotalus atrox
Biochemistry
17
3395-3404
1978
-
-
-
-
-
-
2
-
-
2
3
1
-
2
-
1
1
-
-
2
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5
1
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2
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2
3
1
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1
1
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2
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5
1
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