Application | Comment | Organism |
---|---|---|
medicine | one of the most hemorrhagic metalloproteinases of the P-II class in the venom, causes extensive hemorrhage in muscle tissue and extensive degeneration of capillaries, degradation of basement membrane proteins and surrounding stroma, its free disintegrin domain possesses platelet-aggregation inhibitory activity | Crotalus atrox |
Cloned (Comment) | Organism |
---|---|
produced with a CMV expression vector in 293 cells | Crotalus atrox |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
alpha2-Macroglobulin | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
antihemorrhagins from serum of Crotalus atrox | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
EDTA | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
synthetic carboxyalkyl peptide | effective inhibitor | Crotalus atrox | |
venom-derived pyroglutamyl-containing peptide | effective inhibitor | Crotalus atrox |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | low concentrations stabilize proteolytic activity | Crotalus atrox | |
Zn2+ | can be replaced by Co2+, which does not significantly alter the proteolytic activity | Crotalus atrox |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25700 | - |
SDS-PAGE | Crotalus atrox |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crotalus atrox | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ion-exchange chromatography and gel filtration | Crotalus atrox |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Crotalus atrox | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
basement membrane preperation from Engel-breth-Holm-Swarm tumor + H2O | great proteolytic activity | Crotalus atrox | ? | - |
? | |
collagen type IV + H2O | - |
Crotalus atrox | ? | - |
? | |
Dimethylcasein + H2O | - |
Crotalus atrox | ? | - |
? | |
Fibrinogen + H2O | rapid cleavage of the Aalpha chain followed by the Bbeta and gamma chains | Crotalus atrox | ? | - |
? | |
Laminin + H2O | - |
Crotalus atrox | ? | - |
? | |
additional information | no cleavage of fibrin | Crotalus atrox | ? | - |
? | |
Nidogen + H2O | - |
Crotalus atrox | ? | - |
? | |
o-aminobenzoyl-Ala-Gly-Leu-Ala-nitrobenzylamide + H2O | - |
Crotalus atrox | ? | - |
? | |
Oxidized insulin B-chain + H2O | - |
Crotalus atrox | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Crotalus atrox |
Synonyms | Comment | Organism |
---|---|---|
Hemorrhagic toxin e | - |
Crotalus atrox |
HT-e | - |
Crotalus atrox |