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show all sequences of 3.4.24.44

Studies on the mechanism of hemorrhage production by five proteolytic hemorrhagic toxins from Crotalus atrox venom

Bjarnason, J.B.; Hamilton, D.; Fox, J.W.; Biol. Chem. Hoppe-Seyler 369, 121-129 (1988)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Crotalus atrox
EDTA
-
Crotalus atrox
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
1 mol zinc/mol enzyme; zinc metalloproteinase
Crotalus atrox
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25700
-
1 * 25700, Crotalus atrox, SDS-PAGE
Crotalus atrox
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Crotalus atrox
-
Western diamondback rattlesnake
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
venom
-
Crotalus atrox
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Basement membrane preparation + H2O
component band a, most active on this substrate
31260
Crotalus atrox
Soluble peptides
-
31260
Crotalus atrox
-
Fibrinogen + H2O
-
31260
Crotalus atrox
Hydrolyzed fibrinogen
non-clotting products
31260
Crotalus atrox
-
Laminin + H2O
basement membrane component, most active on this substrate
31260
Crotalus atrox
?
-
-
-
-
additional information
no hydrolysis of fibrin
31260
Crotalus atrox
?
-
-
-
-
Nidogen + H2O
-
31260
Crotalus atrox
?
-
-
-
-
Oxidized insulin B-chain + H2O
-
31260
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 25700, Crotalus atrox, SDS-PAGE
Crotalus atrox
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Crotalus atrox
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.6
Crotalus atrox
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Crotalus atrox
EDTA
-
Crotalus atrox
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
1 mol zinc/mol enzyme; zinc metalloproteinase
Crotalus atrox
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25700
-
1 * 25700, Crotalus atrox, SDS-PAGE
Crotalus atrox
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
venom
-
Crotalus atrox
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Basement membrane preparation + H2O
component band a, most active on this substrate
31260
Crotalus atrox
Soluble peptides
-
31260
Crotalus atrox
-
Fibrinogen + H2O
-
31260
Crotalus atrox
Hydrolyzed fibrinogen
non-clotting products
31260
Crotalus atrox
-
Laminin + H2O
basement membrane component, most active on this substrate
31260
Crotalus atrox
?
-
-
-
-
additional information
no hydrolysis of fibrin
31260
Crotalus atrox
?
-
-
-
-
Nidogen + H2O
-
31260
Crotalus atrox
?
-
-
-
-
Oxidized insulin B-chain + H2O
-
31260
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 25700, Crotalus atrox, SDS-PAGE
Crotalus atrox
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Crotalus atrox
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.6
Crotalus atrox
Other publictions for EC 3.4.24.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668847
Fox
-
Atrolysin E ...
Crotalus atrox
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
674-676
2004
-
1
1
-
-
-
6
-
-
2
1
-
-
1
-
-
1
-
-
1
-
-
9
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
6
-
-
-
2
1
-
-
-
-
1
-
1
-
-
9
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649412
Shimokawa
Isolation, sequence analysis, ...
Crotalus atrox
Arch. Biochem. Biophys.
354
239-246
1998
-
-
1
-
-
-
-
-
-
-
5
-
-
4
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31259
Fox
Atrolysins: metalloproteinases ...
Crotalus atrox
Methods Enzymol.
248
368-387
1995
-
-
1
-
-
-
6
-
-
2
1
1
-
1
-
1
1
-
-
1
-
-
18
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
-
2
1
1
-
-
1
1
-
1
-
-
18
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
31260
Bjarnason
Studies on the mechanism of he ...
Crotalus atrox
Biol. Chem. Hoppe-Seyler
369
121-129
1988
-
-
-
-
-
-
2
-
-
1
1
-
-
1
-
-
-
-
-
1
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
1
-
-
-
-
-
-
1
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
31276
Bjarnason
Proteolytic specificity and co ...
Crotalus atrox
Biochemistry
22
3770-3778
1983
-
-
-
-
-
-
2
-
-
2
-
-
-
2
-
-
-
-
-
2
-
-
5
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
2
-
-
5
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
31261
Bjarnason
Hemorrhagic toxins from Wester ...
Crotalus atrox
Biochemistry
17
3395-3404
1978
-
-
-
-
-
-
2
-
-
2
3
1
-
2
-
1
1
-
-
2
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
3
1
-
-
1
1
-
2
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-