BRENDA - Enzyme Database show
show all sequences of 3.4.24.44

Atrolysins: metalloproteinases from Crotalus atrox venom

Fox, J.W.; Bjarnason, J.B.; Methods Enzymol. 248, 368-387 (1995)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Crotalus atrox
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Crotalus atrox
EDTA
-
Crotalus atrox
Human plasma alpha2-macroglobulin
-
Crotalus atrox
additional information
synthetic peptide inhibitors with N-[1-(R,S)-3-dicarboxypropyl] moiety
Crotalus atrox
Pyroglutamate peptides
e.g. pyro-Glu-Asn-Trp or pyro-Glu-Glu-Trp
Crotalus atrox
Synthetic peptide inhibitors with N-[1-(R,S)-carboxypentyl] moiety
-
Crotalus atrox
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
can be directly exchanged for zinc by dialysis without loss of specificity or activity
Crotalus atrox
Zn2+
1 mol zinc/mol enzyme; zinc metalloproteinase
Crotalus atrox
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25700
-
1 * 25700, Crotalus atrox, SDS-PAGE
Crotalus atrox
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Basement membranes surrounding capillaries + H2O
Crotalus atrox
contributes to hemorrhagic property by weakening capillary walls, together with atrolysins B and C member of the class P-I hemorrhagic toxins
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Crotalus atrox
-
Western diamondback rattlesnake
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
no glycoprotein
Crotalus atrox
Purification (Commentary)
Commentary
Organism
from lyophilized crude venom
Crotalus atrox
Source Tissue
Source Tissue
Commentary
Organism
Textmining
venom
-
Crotalus atrox
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Basement membrane preparation + H2O
-
31259
Crotalus atrox
Soluble peptides
-
-
-
-
Basement membranes surrounding capillaries + H2O
contributes to hemorrhagic property by weakening capillary walls, together with atrolysins B and C member of the class P-I hemorrhagic toxins
31259
Crotalus atrox
?
-
-
-
-
Collagen type IV alphaI(IV) chains + H2O
basement membrane component, cleavage at Ala219-Gln220
31259
Crotalus atrox
?
-
-
-
-
Collagen type IV alphaII(IV) chain + H2O
basement membrane component, cleavage at Thr228-Leu229
31259
Crotalus atrox
?
-
-
-
-
Fibrinogen + H2O
-
31259
Crotalus atrox
Hydrolyzed fibrinogen
non-clotting products
31259
Crotalus atrox
-
Fibronectin + H2O
-
31259
Crotalus atrox
Hydrolyzed fibronectin
lower MW fragments
31259
Crotalus atrox
-
Gelatin of collagen type I + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Gelatin of collagen type II + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Gelatin of collagen type III + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Gelatin of collagen type V + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Human alpha2-macroglobulin + H2O
cleavage at Val689-Met690 and Gly693-His694
31259
Crotalus atrox
?
-
-
-
-
Laminin + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Laminin/nidogen complex + H2O
cleavage at positions 322, 336, 351, 840 and 953
31259
Crotalus atrox
?
-
-
-
-
additional information
no hydrolysis of fibrin
31259
Crotalus atrox
?
-
-
-
-
additional information
no hydrolysis of native collagens
31259
Crotalus atrox
?
-
-
-
-
additional information
substrate digestion patterns differ from those of atrolysins A and C
31259
Crotalus atrox
?
-
-
-
-
Nidogen + H2O
basement membrane component, cleavage at positions 75, 296, 336, 402, 478, 625, 702 and 920
31259
Crotalus atrox
?
-
-
-
-
Oxidized insulin B-chain + H2O
-
31259
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 25700, Crotalus atrox, SDS-PAGE
Crotalus atrox
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.6
Crotalus atrox
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Crotalus atrox
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Crotalus atrox
EDTA
-
Crotalus atrox
Human plasma alpha2-macroglobulin
-
Crotalus atrox
additional information
synthetic peptide inhibitors with N-[1-(R,S)-3-dicarboxypropyl] moiety
Crotalus atrox
Pyroglutamate peptides
e.g. pyro-Glu-Asn-Trp or pyro-Glu-Glu-Trp
Crotalus atrox
Synthetic peptide inhibitors with N-[1-(R,S)-carboxypentyl] moiety
-
Crotalus atrox
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
can be directly exchanged for zinc by dialysis without loss of specificity or activity
Crotalus atrox
Zn2+
1 mol zinc/mol enzyme; zinc metalloproteinase
Crotalus atrox
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25700
-
1 * 25700, Crotalus atrox, SDS-PAGE
Crotalus atrox
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Basement membranes surrounding capillaries + H2O
Crotalus atrox
contributes to hemorrhagic property by weakening capillary walls, together with atrolysins B and C member of the class P-I hemorrhagic toxins
?
-
-
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
no glycoprotein
Crotalus atrox
Purification (Commentary) (protein specific)
Commentary
Organism
from lyophilized crude venom
Crotalus atrox
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
venom
-
Crotalus atrox
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Basement membrane preparation + H2O
-
31259
Crotalus atrox
Soluble peptides
-
-
-
-
Basement membranes surrounding capillaries + H2O
contributes to hemorrhagic property by weakening capillary walls, together with atrolysins B and C member of the class P-I hemorrhagic toxins
31259
Crotalus atrox
?
-
-
-
-
Collagen type IV alphaI(IV) chains + H2O
basement membrane component, cleavage at Ala219-Gln220
31259
Crotalus atrox
?
-
-
-
-
Collagen type IV alphaII(IV) chain + H2O
basement membrane component, cleavage at Thr228-Leu229
31259
Crotalus atrox
?
-
-
-
-
Fibrinogen + H2O
-
31259
Crotalus atrox
Hydrolyzed fibrinogen
non-clotting products
31259
Crotalus atrox
-
Fibronectin + H2O
-
31259
Crotalus atrox
Hydrolyzed fibronectin
lower MW fragments
31259
Crotalus atrox
-
Gelatin of collagen type I + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Gelatin of collagen type II + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Gelatin of collagen type III + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Gelatin of collagen type V + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Human alpha2-macroglobulin + H2O
cleavage at Val689-Met690 and Gly693-His694
31259
Crotalus atrox
?
-
-
-
-
Laminin + H2O
-
31259
Crotalus atrox
?
-
-
-
-
Laminin/nidogen complex + H2O
cleavage at positions 322, 336, 351, 840 and 953
31259
Crotalus atrox
?
-
-
-
-
additional information
no hydrolysis of fibrin
31259
Crotalus atrox
?
-
-
-
-
additional information
no hydrolysis of native collagens
31259
Crotalus atrox
?
-
-
-
-
additional information
substrate digestion patterns differ from those of atrolysins A and C
31259
Crotalus atrox
?
-
-
-
-
Nidogen + H2O
basement membrane component, cleavage at positions 75, 296, 336, 402, 478, 625, 702 and 920
31259
Crotalus atrox
?
-
-
-
-
Oxidized insulin B-chain + H2O
-
31259
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 25700, Crotalus atrox, SDS-PAGE
Crotalus atrox
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 5.6
Crotalus atrox
Other publictions for EC 3.4.24.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668847
Fox
-
Atrolysin E ...
Crotalus atrox
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
674-676
2004
-
1
1
-
-
-
6
-
-
2
1
-
-
1
-
-
1
-
-
1
-
-
9
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
6
-
-
-
2
1
-
-
-
-
1
-
1
-
-
9
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649412
Shimokawa
Isolation, sequence analysis, ...
Crotalus atrox
Arch. Biochem. Biophys.
354
239-246
1998
-
-
1
-
-
-
-
-
-
-
5
-
-
4
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31259
Fox
Atrolysins: metalloproteinases ...
Crotalus atrox
Methods Enzymol.
248
368-387
1995
-
-
1
-
-
-
6
-
-
2
1
1
-
1
-
1
1
-
-
1
-
-
18
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
-
2
1
1
-
-
1
1
-
1
-
-
18
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
31260
Bjarnason
Studies on the mechanism of he ...
Crotalus atrox
Biol. Chem. Hoppe-Seyler
369
121-129
1988
-
-
-
-
-
-
2
-
-
1
1
-
-
1
-
-
-
-
-
1
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
1
-
-
-
-
-
-
1
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
31276
Bjarnason
Proteolytic specificity and co ...
Crotalus atrox
Biochemistry
22
3770-3778
1983
-
-
-
-
-
-
2
-
-
2
-
-
-
2
-
-
-
-
-
2
-
-
5
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
2
-
-
5
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
31261
Bjarnason
Hemorrhagic toxins from Wester ...
Crotalus atrox
Biochemistry
17
3395-3404
1978
-
-
-
-
-
-
2
-
-
2
3
1
-
2
-
1
1
-
-
2
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
3
1
-
-
1
1
-
2
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-