BRENDA - Enzyme Database
show all sequences of 3.4.24.44

Proteolytic specificity and cobalt exchange of hemorrhagic toxin e, a zinc protease isolated from the venom of the western diamondback rattlesnake (Crotalus atrox)

Bjarnason, J.B.; Fox, J.W.; Biochemistry 22, 3770-3778 (1983)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
insulin A-chain
Crotalus atrox
additional information
no inhibition by aprotinin (insulin A-chain as substrate)
Crotalus atrox
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
can be directly exchanged for zinc by dialysis without loss of specificity or activity; no conformational change accompanies the metal exchange
Crotalus atrox
Zn2+
zinc metalloproteinase; zinc removal causes large structural perturbations replacing it with Co2+ is accomplished without structural changes
Crotalus atrox
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Crotalus atrox
-
Western diamondback rattlesnake
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
venom
-
Crotalus atrox
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Dimethylcasein + H2O
-
31276
Crotalus atrox
?
-
-
-
-
Oxidized insulin A-chain + H2O
rapid cleavage at Tyr14-Gln15, more slowly at Ala8-Ser9, kinetics
31276
Crotalus atrox
Hydrolyzed oxidized insulin A-chain
peptides (Gln15-Asn21) + (Gly1-Tyr14) + (Ser9-Tyr14) + (Gly1-Ala8)
31276
Crotalus atrox
-
Oxidized insulin B-chain + H2O
cleavage sites: Ser9-His10, Ala14-Leu15 (very rapidly)
31276
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Oxidized insulin B-chain + H2O
kinetics
31276
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Oxidized insulin B-chain + H2O
cleavage sites: Asn3-Gln4 (very slowly)
31276
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Crotalus atrox
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00767
-
oxidized insulin A-chain
Ala8-Ser9 bond
Crotalus atrox
0.0433
-
oxidized insulin A-chain
Tyr14-Gln15 bond, cobaltous toxin
Crotalus atrox
0.06
-
oxidized insulin A-chain
Tyr14-Gln15 bond
Crotalus atrox
0.167
-
dimethylcasein
cobaltous toxin
Crotalus atrox
0.217
-
dimethylcasein
-
Crotalus atrox
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
insulin A-chain
Crotalus atrox
additional information
no inhibition by aprotinin (insulin A-chain as substrate)
Crotalus atrox
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
can be directly exchanged for zinc by dialysis without loss of specificity or activity; no conformational change accompanies the metal exchange
Crotalus atrox
Zn2+
zinc metalloproteinase; zinc removal causes large structural perturbations replacing it with Co2+ is accomplished without structural changes
Crotalus atrox
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
venom
-
Crotalus atrox
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Dimethylcasein + H2O
-
31276
Crotalus atrox
?
-
-
-
-
Oxidized insulin A-chain + H2O
rapid cleavage at Tyr14-Gln15, more slowly at Ala8-Ser9, kinetics
31276
Crotalus atrox
Hydrolyzed oxidized insulin A-chain
peptides (Gln15-Asn21) + (Gly1-Tyr14) + (Ser9-Tyr14) + (Gly1-Ala8)
31276
Crotalus atrox
-
Oxidized insulin B-chain + H2O
cleavage sites: Ser9-His10, Ala14-Leu15 (very rapidly)
31276
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Oxidized insulin B-chain + H2O
kinetics
31276
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Oxidized insulin B-chain + H2O
cleavage sites: Asn3-Gln4 (very slowly)
31276
Crotalus atrox
Hydrolyzed oxidized insulin B-chain
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Crotalus atrox
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00767
-
oxidized insulin A-chain
Ala8-Ser9 bond
Crotalus atrox
0.0433
-
oxidized insulin A-chain
Tyr14-Gln15 bond, cobaltous toxin
Crotalus atrox
0.06
-
oxidized insulin A-chain
Tyr14-Gln15 bond
Crotalus atrox
0.167
-
dimethylcasein
cobaltous toxin
Crotalus atrox
0.217
-
dimethylcasein
-
Crotalus atrox
Other publictions for EC 3.4.24.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668847
Fox
-
Atrolysin E ...
Crotalus atrox
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
674-676
2004
-
1
1
-
-
-
6
-
-
2
1
-
-
1
-
-
1
-
-
1
-
-
9
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
6
-
-
-
2
1
-
-
-
-
1
-
1
-
-
9
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649412
Shimokawa
Isolation, sequence analysis, ...
Crotalus atrox
Arch. Biochem. Biophys.
354
239-246
1998
-
-
1
-
-
-
-
-
-
-
5
-
-
4
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31259
Fox
Atrolysins: metalloproteinases ...
Crotalus atrox
Methods Enzymol.
248
368-387
1995
-
-
1
-
-
-
6
-
-
2
1
1
-
1
-
1
1
-
-
1
-
-
18
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
-
2
1
1
-
-
1
1
-
1
-
-
18
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
31260
Bjarnason
Studies on the mechanism of he ...
Crotalus atrox
Biol. Chem. Hoppe-Seyler
369
121-129
1988
-
-
-
-
-
-
2
-
-
1
1
-
-
1
-
-
-
-
-
1
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
1
-
-
-
-
-
-
1
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
31276
Bjarnason
Proteolytic specificity and co ...
Crotalus atrox
Biochemistry
22
3770-3778
1983
-
-
-
-
-
-
2
-
-
2
-
-
-
2
-
-
-
-
-
2
-
-
5
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
2
-
-
5
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
31261
Bjarnason
Hemorrhagic toxins from Wester ...
Crotalus atrox
Biochemistry
17
3395-3404
1978
-
-
-
-
-
-
2
-
-
2
3
1
-
2
-
1
1
-
-
2
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
3
1
-
-
1
1
-
2
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-