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Literature summary for 3.4.24.43 extracted from
- Purification and biochemical characterization of atroxase, a nonhemorrhagic fibrinolytic protease from western diamondback rattlesnake venom (1988), Biochemistry, 27, 4769-4777.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha2-Macroglobulin | - |
Crotalus atrox | |
| EDTA | Zn2+ protects | Crotalus atrox |  |
| additional information | no inhibition by aprotinin, PMSF or soybean trypsin inhibitor | Crotalus atrox |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.3 mol/mol enzyme | Crotalus atrox | |
| K+ | 1 mol/mol enzyme | Crotalus atrox | |
| Zn2+ | class I zinc metalloproteinase, contains 1 mol zinc/mol enzyme, no activation by added Zn2+ | Crotalus atrox | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Crotalus atrox |
| 23500 | - |
x * 23500, Crotalus atrox, SDS-PAGE | Crotalus atrox |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Crotalus atrox | nontoxic venom component, nonhemorrhagic endopeptidase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crotalus atrox | - |
Western diamond rattlesnake | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from lyophilized crude venom | Crotalus atrox |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Crotalus atrox | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1.67 mg fibrinogen/min mg enzyme | Crotalus atrox |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Dimethylcasein + H2O | - |
Crotalus atrox | ? | - |
? | |
| Human fibrinogen + H2O | gamma chain is not hydrolyzed | Crotalus atrox | ? | - |
? | |
| Human fibrinogen + H2O | hydrolysis of Aalpha and Bbeta chain, cleavage pattern different from thrombin cleavage pattern | Crotalus atrox | ? | - |
? | |
| additional information | no hemorrhagic activity, no substrates are thrombin, synthetic substrate for thrombin S-2238 | Crotalus atrox | ? | - |
? | |
| additional information | nontoxic venom component, nonhemorrhagic endopeptidase | Crotalus atrox | ? | - |
? | |
| Oxidized insulin B-chain | cleavage of His5-Leu6, Ser9-His10, His10-Leu11, Ala14-Leu15, Tyr16-Leu17 | Crotalus atrox | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23500, Crotalus atrox, SDS-PAGE | Crotalus atrox |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Crotalus atrox |
| 55 | - |
- |
Crotalus atrox |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
inactivation | Crotalus atrox |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 9.6 | Crotalus atrox |
| 9 | - |
- |
Crotalus atrox |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 11 | - |
inactivation | Crotalus atrox |
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