Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.40 extracted from

  • Morihara, K.
    Pseudomonas aeruginosa proteinase. I. Purification and general properties (1963), Biochim. Biophys. Acta, 73, 113-124.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
Pseudomonas aeruginosa Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 10 mM; strong Pseudomonas aeruginosa
2,2'-bipyridyl not Pseudomonas aeruginosa
Cu2+
-
Pseudomonas aeruginosa
EDTA Co2+; Mn2+; strong, 5 mM Pseudomonas aeruginosa
Fe2+ strong Pseudomonas aeruginosa
Hg2+
-
Pseudomonas aeruginosa
KCN not Pseudomonas aeruginosa
KMnO4
-
Pseudomonas aeruginosa
additional information citrate, NaF, oxalate; diisopropyl phosphofluoridate; monoiodoacetic acid; PCMB; potato inhibitor; soybean trypsin inhibitor Pseudomonas aeruginosa
N-bromosuccinimide
-
Pseudomonas aeruginosa
Pb2+
-
Pseudomonas aeruginosa
Sodium thioglycolate not Pseudomonas aeruginosa
Urea
-
Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Pseudomonas aeruginosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Pseudomonas aeruginosa ?
-
?
Egg albumin + H2O
-
Pseudomonas aeruginosa ?
-
?
Gelatin + H2O
-
Pseudomonas aeruginosa ?
-
?
Hemoglobin + H2O
-
Pseudomonas aeruginosa ?
-
?
additional information benzoyl-L-Arg amide, acetyl-L-Tyr ethyl ester, carbobenzoxy-Glu-Tyr, carbobenzoxy-Gly-Phe, L-Leu amide Pseudomonas aeruginosa ?
-
?
additional information collagen Pseudomonas aeruginosa ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Pseudomonas aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
42 62 about half-maximal activity at 42°C and 62°C Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 5-9, fairly stable Pseudomonas aeruginosa
50
-
below, 10 min, fairly stable at neutral pH-values Pseudomonas aeruginosa
55
-
10 min, partly unstable in distilled water, 100 mM phosphate or 10 mM CaCl2 stabilizes Pseudomonas aeruginosa
63
-
10 min, inactivation in the presence of stabilizing agents Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 4 Pseudomonas aeruginosa
7 9 egg white albumin, hemoglobin as substrates Pseudomonas aeruginosa
8 9 casein as substrate Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
6 10 about half-maximal activity at pH 6 and about 80% of maximal activity at pH 10, casein as substrate, about 75% of maximal activity at pH 6 and about 60% of maximal activity at pH 10, egg white albumin as substrate Pseudomonas aeruginosa
6.5 10 about half-maximal activity at pH 6.5 and about 65% of maximal activity at pH 10, hemoglobin as substrate Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 11 stable in this range Pseudomonas aeruginosa
5 9 10 min, fairly stable at 30°C Pseudomonas aeruginosa