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Literature summary for 3.4.24.38 extracted from

  • Jaenicke, L.; Kuhne, W.; Spessert, R.; Wahle, U.; Waffenschmidt, S.
    Cell-wall lytic enzymes (autolysins) of Chlamydomonas reinhardtii are (hydroxy)proline-specific proteases (1987), Eur. J. Biochem., 170, 485-491.
    View publication on PubMed
Search for more literature for 3.4.24.38

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.2 mM, strong Chlamydomonas reinhardtii
2,2'-dipyridyl 2 mM Chlamydomonas reinhardtii
Aprotinin
-
 Chlamydomonas reinhardtii
cysteine
-
 Chlamydomonas reinhardtii
EDTA 0.4 mM, partially reversed by Ca2+ or Mg2+ Chlamydomonas reinhardtii
histidine
-
 Chlamydomonas reinhardtii
imidazole
-
 Chlamydomonas reinhardtii
Lectin from Canavalia ensiformis not from Ricinus communis Chlamydomonas reinhardtii
additional information chymostatin, antipain, leupeptin; pepstatin A Chlamydomonas reinhardtii
phosphoramidon weak Chlamydomonas reinhardtii
PMSF
-
 Chlamydomonas reinhardtii
tosyl-Lys chloromethyl ketone 2 mM, less efficient than tosyl-Phe-chloromethyl ketone Chlamydomonas reinhardtii
tosyl-Phe chloromethyl ketone i.e. TPCK, 2 mM Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 Chlamydomonas reinhardtii, gel filtration Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
 green alga, wild-type strain 137c
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Chlamydomonas reinhardtii

Purification (Commentary)

Purification (Comment) Organism
-
 Chlamydomonas reinhardtii

Source Tissue

Source Tissue Comment Organism Textmining
additional information isolated from gametes Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Azocoll + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
Gelatin + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
Heat-denatured collagen + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
Leu-Trp-Met-Arg-Phe-Ala + H2O
-
 Chlamydomonas reinhardtii Leu-Trp-Met + Arg-Phe-Ala + Leu-Trp + Met-Arg-Phe-Ala
-
 ?
additional information bovine serum albumin Chlamydomonas reinhardtii ?
-
 ?
additional information hemoglobin, casein, poly(Pro) or poly(hydroxyproline) Chlamydomonas reinhardtii ?
-
 ?
additional information Chlamydomonas reinhardtii has a second cell wall lytic enzyme, a sporangial autolysin Chlamydomonas reinhardtii ?
-
 ?
Proteins rich in proline and hydroxyproline + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
Zoosporangia cell walls + H2O selected domains within walls of gametes, zoospores or zoosporangia Chlamydomonas reinhardtii ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 67000, Chlamydomonas reinhardtii, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
Gamete autolysin
-
 Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 inactivation Chlamydomonas reinhardtii
50
-
 30 min Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 broad Chlamydomonas reinhardtii

pH Range

pH Minimum pH Maximum Comment Organism
6 11 about half-maximal activity at pH 6 and 11, about 80% of maximal activity at pH 7 and 10.5 Chlamydomonas reinhardtii

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
 several h stable Chlamydomonas reinhardtii