Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.35 extracted from

  • George, H.J.; Marchand, P.; Murphy, K.; Wiswall, B.H.; Dowling, R.; giannaras, J.; Hollis, G.F.; Trzaskos, J.M.; Copeland, R.A.
    Recombinant human 92-kDa type IV collagenase/gelatinase from baculovirus-infected insect cells: expression, purification, and characterization (1997), Protein Expr. Purif., 10, 154-161.
    View publication on PubMed
Search for more literature for 3.4.24.35

Activating Compound

Activating Compound Comment Organism Structure
APMA
-
 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in baculovirus-infected Spodoptera frugiperda IPLB-Sf21 AE cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00422
-
 N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-7-methoxycoumarin-4-yl acetyl pH 7.6, 25°C, wild type Homo sapiens
0.00496
-
 N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-7-methoxycoumarin-4-yl acetyl pH 7.6, 25°C, recombinant enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ active-site zinc-binding domain Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, stored in 50% glycerol, complete retention of activity forat least 1 month Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-7-methoxycoumarin-4-yl acetyl + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Type IV collagenase/gelatinase
-
 Homo sapiens