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Literature summary for 3.4.24.34 extracted from

  • Marini, S.; Fasciglione, G.F.; de Sanctis, G.; D'Alessio, S.; Politi, V.; Coletta, M.
    Cleavage of bovine collagen I by neutrophil collagenase MMP-8. Effect of pH on the catalytic properties as compared to synthetic substrates (2000), J. Biol. Chem., 275, 18657-18663.
    View publication on PubMed
Search for more literature for 3.4.24.34

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000208
-
 bovine collagen I pH 7.0, 37°C, 1-protonated substrate Homo sapiens
0.0184
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminipropionyl-Ala-Arg-NH2 pH 7.0, 37°C, 1-protonated and 2-protonated substrate Homo sapiens
0.0208
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-DIAMIONOPROPIONYL-Ala-Arg-NH2 pH 7.0, 37°C, unprotonated substrate Homo sapiens
0.0265
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 7.0, 37°C, 3-protonated substrate Homo sapiens
0.0279
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly-norvaline-His-Ala-Arg-NH2 pH 7.0, 37°C, 3-protonated substrate Homo sapiens
0.0315
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly-norvaline-His-Ala-Arg-NH2 pH 7.0, 37°C, 4-protonated substrate Homo sapiens
0.0457
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly-norvaline-His-Ala-Arg-NH2 pH 7.0, 37°C, unprotonated, 1-protonated and 2-protonated substrate Homo sapiens
0.046
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 7.0, 37°C, 4-protonated and 5-protonated substrate Homo sapiens
0.0622
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly-norvaline-His-Ala-Arg-NH2 pH 7.0, 37°C, 5-protonated substrate Homo sapiens
0.102
-
 bovine collagen I pH 7.0, 37°C, 4-protonated and 5-protonated substrate Homo sapiens
0.128
-
 bovine collagen I pH 7.0, 37°C, 2-protonated substrate Homo sapiens
0.958
-
 bovine collagen I pH 7.0, 37°C, unprotonated substrate Homo sapiens
14.4
-
 bovine collagen I pH 7.0, 37°C, 3-protonated substrate Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly-norvaline-His-Ala-Arg-NH2 + H2O
-
 Homo sapiens (7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly + Nva-His-Ala-Arg-NH2
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminipropionyl-Ala-Arg-NH2 + H2O
-
 Homo sapiens ?
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 + H2O
-
 Homo sapiens ?
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-DIAMIONOPROPIONYL-Ala-Arg-NH2 + H2O
-
 Homo sapiens ?
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 + H2O
-
 Homo sapiens (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly + Leu-N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2
-
 ?
bovine collagen I + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
MMP-8
-
 Homo sapiens
neutrophil collagenase
-
 Homo sapiens
neutrophil collagenase MMP-8
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0109
-
 bovine collagen I pH 7.0, 37°C, 4-protonated and 5-protonated substrate Homo sapiens
0.47
-
 bovine collagen I pH 7.0, 37°C, unprotonated and 1-protonated substrate Homo sapiens
15
-
 bovine collagen I pH 7.0, 37°C, 2-protonated substrate Homo sapiens
148
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 7.0, 37°C, 5-protonated substrate Homo sapiens
164
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 7.0, 37°C, unprotonated, 1-protonated and 2-protonated substrate Homo sapiens
180
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly-norvaline-His-Ala-Arg-NH2 pH 7.0, 37°C, 5-protonated substrate Homo sapiens
246
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly-norvaline-His-Ala-Arg-NH2 pH 7.0, 37°C, unprotonated, 1-protonated and 2-protonated substrate Homo sapiens
250
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly-norvaline-His-Ala-Arg-NH2 pH 7.0, 37°C, 3-protonated substrate Homo sapiens
324
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 7.0, 37°C, 3-protonated substrate Homo sapiens
562
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 7.0, 37°C, 4-protonated substrate Homo sapiens
570
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-cyclohexylalanine-Gly-norvaline-His-Ala-Arg-NH2 pH 7.0, 37°C, 4-protonated substrate Homo sapiens
1120
-
 bovine collagen I pH 7.0, 37°C, 3-protonated substrate Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
 Homo sapiens