Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate buffer | - |
Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol | Vibrio sp. |
28000 | - |
x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol | Vibrio sp. |
105000 | - |
gel filtration | Vibrio sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Collagen + H2O | Vibrio sp. | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | ? | - |
? | |
Collagen + H2O | Vibrio sp. B-30 | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | - |
B-30 | - |
Vibrio sp. B-30 | - |
B-30 | - |
Purification (Comment) | Organism |
---|---|
partial | Vibrio sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Vibrio sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Vibrio sp. |
Storage Stability | Organism |
---|---|
4°C, 24 h, pH 4.4-7.4 | Vibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form | Vibrio sp. | Hydrolyzed collagen | - |
? | |
Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form | Vibrio sp. B-30 | Hydrolyzed collagen | - |
? | |
Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | Vibrio sp. | ? | - |
? | |
Collagen + H2O | degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro | Vibrio sp. B-30 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol | Vibrio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Vibrio sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.4 | 7.4 | 4°C, 24 h | Vibrio sp. |