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Literature summary for 3.4.24.3 extracted from

  • Hanada, K.; Mizutani, T.; Yamagishi, M.; Tsuji, H.; Misaki, T.; Sawada, J.
    The isolation of collagenase and its enzymological and physico-chemical proterties (1973), Agric. Biol. Chem., 37, 1771-1781.
No PubMed abstract available
Search for more literature for 3.4.24.3

General Stability

General Stability Organism
Ca2+ stabilizes Pseudoalteromonas mariniglutinosa

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Pseudoalteromonas mariniglutinosa
NaCN
-
 Pseudoalteromonas mariniglutinosa
SDS surface active substance Pseudoalteromonas mariniglutinosa
Sodiumthioglycolate
-
 Pseudoalteromonas mariniglutinosa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Pseudoalteromonas mariniglutinosa
Ca2+ 4 g-atom Calcium per mol enzyme Pseudoalteromonas mariniglutinosa
Co2+ activates at low concentration, inhibits at high concentration Pseudoalteromonas mariniglutinosa
Mg2+ activates Pseudoalteromonas mariniglutinosa
Mn2+ activates Pseudoalteromonas mariniglutinosa
Sr2+ activates Pseudoalteromonas mariniglutinosa
Zn2+ 1 mol zinc per mol enzyme Pseudoalteromonas mariniglutinosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 gel filtration Pseudoalteromonas mariniglutinosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Pseudoalteromonas mariniglutinosa degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas mariniglutinosa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Pseudoalteromonas mariniglutinosa

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudoalteromonas mariniglutinosa

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Pseudoalteromonas mariniglutinosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudoalteromonas mariniglutinosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbobenzoxy-Gly-Pro-Leu-Gly-Pro + H2O
-
 Pseudoalteromonas mariniglutinosa carbobenzoxy-Gly-Pro-Leu + Gly-Pro
-
 ?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Pseudoalteromonas mariniglutinosa Hydrolyzed collagen
-
 ?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Pseudoalteromonas mariniglutinosa ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Pseudoalteromonas mariniglutinosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, 40% loss of activity Pseudoalteromonas mariniglutinosa
60
-
 30 min, complete inactivation Pseudoalteromonas mariniglutinosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.6
-
 Pseudoalteromonas mariniglutinosa

pH Range

pH Minimum pH Maximum Comment Organism
4.2 10.2 pH 10.2: about 85% of activity maximum Pseudoalteromonas mariniglutinosa
4.2 10.2 pH 4.2: about 20% of activity maximum Pseudoalteromonas mariniglutinosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2 8.7 unstable outside the range Pseudoalteromonas mariniglutinosa