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Literature summary for 3.4.24.3 extracted from

  • Sugasawara, R.; Harper, E.
    Purification and characterization of three forms of collagenase from Clostridium histolyticum (1984), Biochemistry, 23, 5175-5181.
    View publication on PubMed
Search for more literature for 3.4.24.3

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc enzyme, 1.14 mol zinc per mol, enzyme form C1, 0.82 mol zinc per mol enzyme, form C3 Hathewaya histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 ? * 76000, C3, SDS-PAGE Hathewaya histolytica
92000
-
 ? * 92000, C2, SDS-PAGE Hathewaya histolytica
96000
-
 ? * 96000, C1, SDS-PAGE Hathewaya histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Hathewaya histolytica degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
 ?

Organism

Organism UniProt Comment Textmining
Hathewaya histolytica
-
 3 forms: C1, C2, C3 differ in molecular weight
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification C1: no carbohydrate Hathewaya histolytica

Purification (Commentary)

Purification (Comment) Organism
-
 Hathewaya histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Hathewaya histolytica Hydrolyzed collagen
-
 ?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Hathewaya histolytica ?
-
 ?

Subunits

Subunits Comment Organism
? ? * 76000, C3, SDS-PAGE Hathewaya histolytica
? ? * 92000, C2, SDS-PAGE Hathewaya histolytica
? ? * 96000, C1, SDS-PAGE Hathewaya histolytica