Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.3 extracted from

  • Peterkofsky, B.
    Bacterial collagenases (1982), Methods Enzymol., 82, 453-471.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Vibrio sp. degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
?
Collagen + H2O Hathewaya histolytica degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
?
Collagen + H2O Vibrio sp. B-30 degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
?

Organism

Organism UniProt Comment Textmining
Hathewaya histolytica
-
2-6 different collagenase fractions can be differentiated on the basis of substrate preference
-
Vibrio sp.
-
B-30
-
Vibrio sp. B-30
-
B-30
-

Purification (Commentary)

Purification (Comment) Organism
-
Hathewaya histolytica

Storage Stability

Storage Stability Organism
-20°C, lyophilized enzyme: 5 years, solutions: several months Hathewaya histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg + H2O
-
Hathewaya histolytica 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg
-
?
Azocoll + H2O
-
Hathewaya histolytica Hydrolyzed azocoll
-
?
carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala + H2O
-
Hathewaya histolytica carbobenzoxy-Gly-Pro-Gly + Gly-Pro-Ala
-
?
carbobenzoxy-Gly-Pro-Leu-Gly-Pro + H2O
-
Hathewaya histolytica carbobenzoxy-Gly-Pro-Leu + Gly-Pro
-
?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Vibrio sp. Hydrolyzed collagen
-
?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Hathewaya histolytica Hydrolyzed collagen
-
?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Vibrio sp. B-30 Hydrolyzed collagen
-
?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Vibrio sp. ?
-
?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Hathewaya histolytica ?
-
?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Vibrio sp. B-30 ?
-
?
additional information other forms with broader specificity isolated from the same source: collagenase B, collagenase II, pseudocollagenase Vibrio sp. ?
-
?
additional information other forms with broader specificity isolated from the same source: collagenase B, collagenase II, pseudocollagenase Hathewaya histolytica ?
-
?
additional information minimum sequence requirement: R-Pro-X-Gly-Pro, Pro can be substituted by Hyp and the first imino group must be blocked, cleavage between X and Gly Vibrio sp. ?
-
?
additional information minimum sequence requirement: R-Pro-X-Gly-Pro, Pro can be substituted by Hyp and the first imino group must be blocked, cleavage between X and Gly Hathewaya histolytica ?
-
?
additional information other forms with broader specificity isolated from the same source: collagenase B, collagenase II, pseudocollagenase Vibrio sp. B-30 ?
-
?
additional information minimum sequence requirement: R-Pro-X-Gly-Pro, Pro can be substituted by Hyp and the first imino group must be blocked, cleavage between X and Gly Vibrio sp. B-30 ?
-
?