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Literature summary for 3.4.24.29 extracted from
- Studies on extracellular proteolytic enzymes from Staphylococcus aureus. I. Purification and characterization of one neutral and one alkaline protease (1973), Biochim. Biophys. Acta, 302, 135-148.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Reducing agents | protease II active only in presence of reducing agents such as cysteine, 2-mercaptoethanol or sodium thioglycollate | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | protease II | Staphylococcus aureus |  |
| Co2+ | protease II inactive in presence of, protease I slightly stimulated | Staphylococcus aureus | |
| Cu2+ | protease II | Staphylococcus aureus | |
| DFP | 1.0 mM, 30 min, 20°C, activity of protease I is reduced by 10%, activity of protease II by 30% | Staphylococcus aureus | |
| Hg2+ | protease II | Staphylococcus aureus | |
| Mn2+ | protease II | Staphylococcus aureus | |
| additional information | protease I is not significantly affected by SH-reducing, SH-inactivating or metal-complexing agents | Staphylococcus aureus | |
| NaCl | 50% reduction of activity at 0.3 M for protease I and 0.5 M for protease II | Staphylococcus aureus | |
| Zn2+ | protease II | Staphylococcus aureus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Staphylococcus aureus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight stimulation of protease I | Staphylococcus aureus | |
| Co2+ | slight stimulation of protease I, protease II inactive in presence of | Staphylococcus aureus | |
| Mg2+ | slight stimulation of protease I | Staphylococcus aureus | |
| Pb2+ | slight stimulation of protease I | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12500 | - |
Staphylococcus aureus, protease II, gel filtration | Staphylococcus aureus |
| 21000 | - |
Staphylococcus aureus, protease I, gel filtration | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
| Staphylococcus aureus V8 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protease I (pI: 4.0) and protease II (pI: 9.4) | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Staphylococcus aureus | hydrolyzed casein | - |
? | |
| casein + H2O | - |
Staphylococcus aureus V8 | hydrolyzed casein | - |
? | |
| Hemoglobin + H2O | - |
Staphylococcus aureus | Hydrolyzed hemoglobin | - |
? | |
| Hemoglobin + H2O | - |
Staphylococcus aureus V8 | Hydrolyzed hemoglobin | - |
? | |
| additional information | protease II exhibits esterase activity, using N-benzoyl-L-Tyr ethyl ester as substrate, no activity of protease I | Staphylococcus aureus | ? | - |
? | |
| additional information | protease II exhibits esterase activity, using N-benzoyl-L-Tyr ethyl ester as substrate, no activity of protease I | Staphylococcus aureus V8 | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 4.0-11.0, 3 h, protease I stable | Staphylococcus aureus |
| 45 | - |
pH 7.0, 30 min, stable up to, protease I | Staphylococcus aureus |
| 50 | - |
pH 7.0, 30 min, 90% loss of activity, protease II | Staphylococcus aureus |
| 90 | - |
pH 7.0, 30 min, complete inactivation, protease I | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
and 7.0-9.0, hemoglobin, protease I | Staphylococcus aureus |
| 7 | 9 | and 5.0, hemoglobin, protease I | Staphylococcus aureus |
| 7.8 | - |
casein, protease I | Staphylococcus aureus |
| 8.8 | - |
casein, protease II | Staphylococcus aureus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | 37°C, 3 h, protease I stable | Staphylococcus aureus |
| 7 | - |
37°C, 3 h, 47% loss of activity, protease II | Staphylococcus aureus |
| 8 | - |
37°C, 3 h, 74% loss of activity, protease II | Staphylococcus aureus |
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