Literature summary for 3.4.24.28 extracted from

Bavaro, T.; Cattaneo, G.; Serra, I.; Benucci, I.; Pregnolato, M.; Terreni, M.
Immobilization of neutral protease from Bacillus subtilis for regioselective hydrolysis of acetylated nucleosides application to capecitabine synthesis (2016), Molecules, 21, E1621 .

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Application

Application	Comment	Organism
synthesis	protease N covalently immobilized on agarose can be used for the preparation of acetylated ribonucleosides with only one free hydroxyl group in position C-5', as useful building blocks for the synthesis of monophosphate nucleotides or 5'-deoxyribonucleosides such as Capecitabine	Bacillus subtilis

General Stability

General Stability	Organism
protease N covalently immobilized on agarose is completely stable in presence of co-solvent (methanol, acetone and acetonitrile (10% v/v),) for the regioselective hydrolysis of acetylated nucleosides	Bacillus subtilis

Organic Solvent Stability

Organic Solvent	Comment	Organism
Acetone	10% v/v, protease N covalently immobilized on agarose is completely stable	Bacillus subtilis
acetonitrile	10% v/v, protease N covalently immobilized on agarose is completely stable	Bacillus subtilis
Methanol	10% v/v, protease N covalently immobilized on agarose is completely stable	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-

Synonyms

Synonyms	Comment	Organism
Bacillus subtilis neutral protease	-	Bacillus subtilis
protease N	-	Bacillus subtilis

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Release 2023.1 (January 2023)